Human Corin Antibody

R&D Systems | Catalog # AF2209

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Corin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Corin
Ser67-Asn1042
Accession # Q9Y5Q5

Specificity

Detects human Corin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Corin Antibody

Detection of Human Corin antibody by Western Blot.

Detection of Human Corin by Western Blot.

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # af2209) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Corin at approximately 85 and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Corin antibody in Human Liver by Immunohistochemistry (IHC-P).

Corin in Human Liver.

Corin was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Corin antibody in Human Heart by Immunohistochemistry (IHC-P).

Corin in Human Heart.

Corin was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Human Corin Antibody in ELISA Standard Curve.

Human Corin ELISA Standard Curve.

Recombinant Human Corin protein was serially diluted 2-fold and captured by Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Human Corin ELISA Standard Curve

Human Corin ELISA Standard Curve

Recombinant Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL (Catalog # 3560-ZN) was serially diluted and captured by Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of Corin Ectodomain by Western Blot

Detection of Corin Ectodomain by Western Blot

Corin promoted orofacial BMSCs osteogenic differentiation in vitro. a qRT-PCR analyses of the corin mRNA expression at 0d, 3d, 7d, 14d after osteogenic differentiation. b MeRIP-qPCR results show the m6A modification levels of corin mRNA. c Representative immunoblotting shows the protein expression of corin. d ELISA results show the sCorin levels at different time points during osteogenic differentiation process. e, f The mRNA and protein expression of corin from sh-NC/ sh-CORIN Group. g, h Representative images and quantitative analyses of ALP and ARS staining of BMSCs from sh-NC/ sh-CORIN Group. i Representative immunoblotting shows the protein expression of Runx2/OCN from sh-NC/sh-CORIN Group. j, k The mRNA and protein expression of corin from Vector/oe-CORIN Group. l, m Representative images and quantitative analyses of ALP and ARS staining of BMSCs from Vector/oe-CORIN Group. n Representative immunoblotting shows the protein expression of Runx2/OCN from Vector/oe-CORIN Group. o The sCorin levels at different time points from Vector/oe-CORIN Group. p, q Representative images and quantitative analyses of ALP and ARS staining of BMSCs from Vector/oe-CORIN supernate Group. r Representative immunoblotting shows the protein expression of Runx2/OCN from Vector/ oe-CORIN supernate Group. Data are expressed as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, sh-NC or Vector vs. sh-CORIN or oe-CORIN Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41368-024-00303-1), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Corin Ectodomain by Western Blot

Detection of Corin Ectodomain by Western Blot

METTL7A promoted corin m6A methylation level. a, b Treated with 500 µg/mL Cycloleucine, representative images and quantitative analyses of ALP and ARS staining of BMSCs from Vector/oe-CORIN Group. c Representative immunoblotting shows the protein expression of Runx2/OCN from Vector/oe-CORIN Group. d, e qRT-PCR analyses, as well as representative immunoblotting show corin expression from sh-NC/sh-METTL7A Group. f MeRIP-qPCR results show m6A modification of corin mRNA from sh-NC/sh-METTL7A Group. g Comparation of corin mRNA stability from sh-NC/sh-METTL7A Group. h, i Corin expression from Vector/oe-METTL7A Group. j MeRIP-qPCR results show m6A modification on corin mRNA from Vector/oe-METTL7A Group. k Comparation of corin mRNA stability from Vector/oe-METTL7A Group. l Construct of Wildtype and mutant 3’UTR CORIN plasmids. m METTL7A binding to the CORIN 3′UTR is verified by the dual-luciferase reporter assay. Data are expressed as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, sh-NC or Vector vs. sh-METTL7A or oe-METTL7A. ###P < 0.001, oe-CORIN vs oe-CORIN+Cycloleucine Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41368-024-00303-1), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Corin Ectodomain by Western Blot

Detection of Corin Ectodomain by Western Blot

Corin promoted orofacial BMSCs osteogenic differentiation in vitro. a qRT-PCR analyses of the corin mRNA expression at 0d, 3d, 7d, 14d after osteogenic differentiation. b MeRIP-qPCR results show the m6A modification levels of corin mRNA. c Representative immunoblotting shows the protein expression of corin. d ELISA results show the sCorin levels at different time points during osteogenic differentiation process. e, f The mRNA and protein expression of corin from sh-NC/ sh-CORIN Group. g, h Representative images and quantitative analyses of ALP and ARS staining of BMSCs from sh-NC/ sh-CORIN Group. i Representative immunoblotting shows the protein expression of Runx2/OCN from sh-NC/sh-CORIN Group. j, k The mRNA and protein expression of corin from Vector/oe-CORIN Group. l, m Representative images and quantitative analyses of ALP and ARS staining of BMSCs from Vector/oe-CORIN Group. n Representative immunoblotting shows the protein expression of Runx2/OCN from Vector/oe-CORIN Group. o The sCorin levels at different time points from Vector/oe-CORIN Group. p, q Representative images and quantitative analyses of ALP and ARS staining of BMSCs from Vector/oe-CORIN supernate Group. r Representative immunoblotting shows the protein expression of Runx2/OCN from Vector/ oe-CORIN supernate Group. Data are expressed as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, sh-NC or Vector vs. sh-CORIN or oe-CORIN Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41368-024-00303-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Corin Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Corin DuoSet ELISA Kit (Catalog # DY2209) for convenient development of a sandwich ELISA or the Human Corin Quantikine ELISA Kit (Catalog # DCRN00) for a complete optimized ELISA.

Immunohistochemistry

0.3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver and human heart

Western Blot

1 µg/mL
Sample: Human Heart Tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Corin

Corin is a type II transmembrane serine protease expressed predominantly in cardiomyocytes. It converts pro arterial natriuretic peptide (pro-ANP) to ANP.

Alternate Names

ATC2, Pro-ANP-Converting Enzyme

Entrez Gene IDs

10699 (Human)

Gene Symbol

CORIN

UniProt

Q9Y5Q5

Additional Corin Products

Product Documents for Human Corin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Corin Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Human Corin Antibody

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Protocols

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