Human COX-2 Antibody (495222)

R&D Systems | Catalog # MAB4198

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Western Blot, ELISA Capture, Western Blots

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 495222
View all COX-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human COX-2
Ala18-Ser112 and Gln386-Leu604
Accession # P35354

Specificity

Detects human COX-2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human COX-2 Antibody (495222)

Detection of Human COX‑2 antibody by Western Blot.

Detection of Human COX‑2 by Western Blot.

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human COX-2 Monoclonal Antibody (Catalog # MAB4198), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for COX-2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
COX-2 antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

COX‑2 in A549 Human Cell Line.

COX-2 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human COX-2 Monoclonal Antibody (Catalog # MAB4198) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
COX-2 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

COX‑2 in Human Breast Cancer Tissue.

COX-2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human COX-2 Monoclonal Antibody (Catalog # MAB4198) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human COX-2 by Western Blot

Detection of Human COX-2 by Western Blot

Effect of monocyte-ASC coculture on levels of monocyte subsets, COX-2, PGE2, and EP4. First, monocytes from healthy donors and sepsis patients were cultured alone or cocultured directly with ASCs. The percentage of CD14++CD16+ (a), CD14+CD16++ (b), and CD14++CD16– (c) monocytes in the total monocyte population were determined via flow cytometry. Box and whisker plots represent median (lines within boxes), interquartile range (bounds of boxes), and error bars (upper and lower range); n = 25 for healthy donors and n = 23 for sepsis patients. ***p < 0.001. Then, ASCs and monocytes from sepsis patients were cultured alone, cocultured directly, or cocultured via Transwell for 24 h. Culture supernatants from the above wells were harvested for quantification of PGE2 via ELISA (d). Lysates from different groups were analyzed for COX-2 and EP4 levels via Western blotting. Representative blots and normalized COX-2 levels (e) and EP4 (f) are shown. beta -actin was used as a protein-loading control. Data are expressed as mean ± SEM; n = 5 per group. *p < 0.05, **p < 0.01. ASC adipose-derived mesenchymal stem (stromal) cell, COX-2 cyclooxygenase-2, EP4 prostaglandin E2 receptor 4, MO monocytes, PGE2 prostaglandin E2 Image collected and cropped by CiteAb from the following publication (https://stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0546-x), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human COX-2 Antibody (495222)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Western Blot

1 µg/mL
Sample: LPS-treated human peripheral blood mononuclear cells (PBMC)

Reviewed Applications

Read 4 reviews rated 4.3 using MAB4198 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: COX-2

Cyclooxygenase-2 (COX-2) also known as prostaglandin G/H synthase 2 (PGHS2) is a 70 kDa microsomal enzyme that belongs to the prostaglandin G/H synthase family. It is inducibly-expressed by a number of cell types, including fibroblasts, vascular smooth muscle cells, endothelium, and monocytes. Functionally, COX-2 is a homodimer that catalyzes two steps in the conversion of arachadonic acid to prostaglandin H2. Mature human COX-2 is 587 amino acids (aa) in length and contains one EGF-like domain (aa 18‑55), a potential membrane interacting region (aa 277‑292) and a globular catalytic domain (aa 293‑604). At least one splice form exists that shows an 11 aa substitution for the C-terminal 451 amino acids. Over the amino acid range of the immunogen, human COX-2 shows 83% aa identity to mouse COX-2.

Long Name

Cyclooxygenase 2

Alternate Names

COX2, PGHS-2, PHS-II, PTGS2

Entrez Gene IDs

5743 (Human); 19225 (Mouse); 29527 (Rat)

Gene Symbol

PTGS2

UniProt

P35354 (Human)

Additional COX-2 Products

Product Documents for Human COX-2 Antibody (495222)

Certificate of Analysis

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Product Specific Notices for Human COX-2 Antibody (495222)

For research use only

Citations for Human COX-2 Antibody (495222)

Customer Reviews for Human COX-2 Antibody (495222) (4)

4.3 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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3 Stars
25%
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1 Stars
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Showing  1 - 4 的 4 reviews Showing All
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  • Human COX-2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Human Breast Cancer Tissue
    Species: Human
    Verified Customer | Posted 10/03/2021
    Human COX-2 Antibody (495222) MAB4198
  • Human COX-2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colonic tissue
    Species: Human
    Verified Customer | Posted 09/02/2021
    Human COX-2 Antibody (495222) MAB4198
  • Human COX-2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Melanoma tissue
    Species: Human
    Verified Customer | Posted 11/23/2020
    Human COX-2 Antibody (495222) MAB4198
  • Human COX-2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human Tonsil tissue
    Species: Human
    Verified Customer | Posted 01/16/2020
    cox-2 1:100 on tonsil
    pH 9 antigen retrieval
    Human COX-2 Antibody (495222) MAB4198

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