Human E-Selectin/CD62E Antibody (BBIG-E5) [Biotin]

R&D Systems | Catalog # BBA8

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA Detection (Matched Antibody Pair), Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # BBIG-E5
View all E-Selectin/CD62E Primary Antibodies »

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

This antibody stains COS cells transfected with cDNA for human E-Selectin/CD62E in immunofluorescence experiments. This antibody does not stain COS cells transfected with cDNA for human ICAM-1, PECAM-1 or VCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human E-Selectin/CD62E Antibody (BBIG-E5) [Biotin]

E-Selectin/CD62E antibody in HUVEC Cells by Immunocytochemistry (ICC).

E-Selectin/CD62E in HUVEC Cells.

E-Selectin/CD62E was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) activated with TNF alpha using Mouse Anti-Human E-Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # BBA8) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Human E-Selectin / CD62E ELISA Standard Curve

Human E-Selectin / CD62E ELISA Standard Curve

Recombinant Human E‑Selectin/CD62E (Catalog # ADP1) was serially diluted and captured by Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human E-Selectin/CD62E Antibody (BBIG-E5) [Biotin]

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF alpha

Western Blot

1 µg/mL
Sample: Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES)
under non-reducing conditions only

Human E-Selectin/CD62E Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human E‑Selectin/CD62E Antibody (Catalog # BBA16)
  • Capture Reagent: Human E‑Selectin/CD62E Antibody (Catalog # BBA2)
  • Standard: Recombinant Human E-Selectin/CD62E Protein, CF (Catalog # ADP1)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BBA8 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E-Selectin/CD62E

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

Alternate Names

CD62E, ELAM1, LECAM2, SELE

Entrez Gene IDs

6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)

Gene Symbol

SELE

Additional E-Selectin/CD62E Products

Product Documents for Human E-Selectin/CD62E Antibody (BBIG-E5) [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human E-Selectin/CD62E Antibody (BBIG-E5) [Biotin]

For research use only

Citations for Human E-Selectin/CD62E Antibody (BBIG-E5) [Biotin]

Customer Reviews for Human E-Selectin/CD62E Antibody (BBIG-E5) [Biotin] (1)

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  • Human E-Selectin/CD62E Biotinylated Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: endothelial cells
    Species: Human
    Verified Customer | Posted 10/14/2022
    Human E-Selectin/CD62E Antibody (BBIG-E5) [Biotin] BBA8

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Protocols

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