Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Porcine, Guinea Pig

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all eNOS Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human eNOS

Specificity

Detects human eNOS in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human eNOS Antibody

Detection of Human eNOS antibody by Western Blot.

Detection of Human eNOS by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothlial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eNOS Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eNOS at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunoprecipitation of Human eNOS.

Immunoprecipitation of Human eNOS.

eNOS was immunoprecipitated from lysates (1 x 106cells) of HUVEC human umbilical vein endothelial cells following incubation with 0.3 µg Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). Immunoprecipitated eNOS was detected by Western blot using 1 µg/mL Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). View our recommended buffer recipes for immunoprecipitation.
Detection of Human eNOS antibody by Simple WesternTM.

Detection of Human eNOS by Simple WesternTM.

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for eNOS at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human eNOS by Western Blot

Detection of Human eNOS by Western Blot

Phenolic metabolites upregulate eNOS pathway in response to TNF-alpha. The expression and phosphorylation of eNOS was measured in HUVECs pre-stimulated with C3G and phenolic metabolites for 24 h +/− TNF-alpha for an additional 24 h. (A) Representative graphs for eNOS mRNA. (B) Representative Western blot bands for eNOS. (C) Western blot representative graphs. All data expressed as mean = 3, ±SEM. Significance value was set at # p < 0.05, ## p < 0.01 vs. control and * p < 0.05 versus TNF-alpha. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2218-1989/14/11/613), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human eNOS Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

0.3 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells, see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: eNOS immunoprecipitated HUVEC human umbilical vein endothelial cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: eNOS

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme encoded by the NOS3 gene. Endothelial NOS generates nitric oxide in blood vessels and is involved with regulating vascular tonehttp://en.wikipedia.org/wiki/Vascular_resistance" title="Vascular resistance"> by inhibiting smooth muscle contraction and platelethttp://en.wikipedia.org/wiki/Platelet" title="Platelet"> aggregation. A constitutive calciumhttp://en.wikipedia.org/wiki/Calcium_in_biology" title="Calcium in biology"> dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of the Golgi apparatushttp://en.wikipedia.org/wiki/Golgi_apparatus" title="Golgi apparatus"> within cells.

Long Name

Endothelial Nitric Oxide Synthase

Alternate Names

NOS3

Entrez Gene IDs

4846 (Human); 18127 (Mouse)

Gene Symbol

NOS3

Additional eNOS Products

Product Documents for Human eNOS Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human eNOS Antibody

For research use only

Citations for Human eNOS Antibody

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Protocols

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