Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488-conjugated Antibody
Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry. MCF-7 human cell line was stained with Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB9589G, filled histogram) or irrelevant antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.
ErbB2/Her2 in SK‑BR‑3 Human Cell Line. ErbB2/Her2 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line using Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB9589G, green) at 5 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB1). This antibody can be used in flow cytometry or various assay formats to measure Her2 expression.
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citation for Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
BET inhibition induces vulnerability to MCL1 targeting through upregulation of fatty acid synthesis pathway in breast cancer
Authors: G Yan, A Luna, H Wang, B Bozorgui, X Li, M Sanchez, Z Dereli, N Kahraman, G Kara, X Chen, C Zheng, D McGrail, N Sahni, Y Lu, O Babur, M Cokol, B Lim, B Ozpolat, C Sander, GB Mills, A Korkut
Cell Reports, 2022-09-13;40(11):111304.
Sample Types: Whole Cells
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