ErbB2/Her2 (Research Grade Trastuzumab
Biosimilar) Biotinylated Antibody

Recombinant Monoclonal Antibody For Research Use Only
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Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry.
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ErbB2/Her2 (Research Grade Trastuzumab
Biosimilar) Biotinylated Antibody Summary

Species Reactivity
Detects human ErbB2/Her2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab. This product is for research use only.
Recombinant Monoclonal Human IgG1 Clone # Hu5
Protein A or G purified from cell culture supernatant
Human ErbB2/Her2
Supplied in a saline solution containing BSA and Sodium Azide.


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below
5-25 µg/mL
Live and fixed SK-BR-3 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ErbB2/Her2 antibody in MCF-7 human cell line antibody by Flow Cytometry. View Larger

Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry. MCF-7 human cell line was stained with Biotinylated Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # FAB9589B, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry ErbB2/Her2 antibody in SK-BR-3 Human Cell Line by Immunocytochemistry (ICC). View Larger

ErbB2/Her2 in SK-BR-3 Human Cell Line. ErbB2/Her2 was detected in both live and fixed SK‑BR‑3 human breast cancer cell line and but not detected in fixed U937 human histiocytic lymphoma cell line. SK-BR-3 live cells were incubated for 1 hour at 4°C with Anti-Human ErbB2/Her2 Biotinylated Monoclonal Antibody (FAB9589B) diluted at 5 µg/mL with HBSS. SK-BR-3 and U937 fixed cells were incubated for 2 hours at room temperature with Anti-Human ErbB2/Her2 Biotinylated Monoclonal Antibody (FAB9589B) diluted with antibody diluent. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ErbB2/Her2

Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB1). The biotinylated antibody can be used in flow cytometry or various assay format to measure Her2 expression and can be detected by conjugated streptavidin.

Long Name
Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs
2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names
CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

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Biosimilar) Biotinylated Antibody

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Biosimilar) Biotinylated Antibody
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Biosimilar) Biotinylated Antibody?

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