Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 733930
View all ER beta/NR3A2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ER beta /NR3A2
Met1-Gly318
Accession # Q92731

Specificity

Detects human ER beta /NR3A2 in direct ELISAs and Western blot. In direct ELISA and Western blot, no cross-reactivity with recombinant human ER alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ER beta /NR3A2 Antibody

Detection of Human ER beta /NR3A2 antibody by Western Blot.

Detection of Human ER beta /NR3A2 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, Saos-2 human osteosarcoma cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB7106) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ER beta /NR3A2 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ER beta /NR3A2 antibody in LNCaP Human Cell Line by Immunocytochemistry (ICC).

ER beta /NR3A2 in LNCaP Human Cell Line.

ER beta /NR3A2 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB7106) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ER beta /NR3A2 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

ER beta /NR3A2 in MCF‑7 Human Cell Line.

ER beta /NR3A2 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB7106) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of ER beta /NR3A2 antibody in MCF‑7 Human Cell Line antibody by Flow Cytometry.

Detection of ER beta /NR3A2 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB7106, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of ER beta /NR3A2 by Western Blot

Detection of ER beta /NR3A2 by Western Blot

Western blot analysis showing the protein expression patterns of the estrogen receptors (ERs) ER alpha (66 kDa) and ER beta (48 kDa). (a) The murine brain endothelial cell lines cEND (left) and the human brain endothelial cell line hCMEC/D3 (right) show the presence of both ERs in three experimental runs (1, 2, 3). (b) The breast cancer (BC) cell lines showed the presence of ER beta in MCF-7, BT-474 and MDA-MB-231 cells, whereas ER alpha was only detected in MCF-7 and BT-474. Again, three experimental runs were performed (1, 2, 3). beta -actin (42 kDa) was used as a loading control. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/6/3379), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human and Mouse ER beta /NR3A2 by Simple WesternTM.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, T47D human breast cancer cell line, MDA‑MB‑231 human breast cancer cell line, and 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for ER beta /NR3A2 at approximately 54 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB7106). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human ER beta /NR3A2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: MCF‑7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Simple Western

50 µg/mL
Sample: MCF‑7 human breast cancer cell line, T47D human breast cancer cell line, MDA‑MB‑231 human breast cancer cell line, and 3T3‑L1 mouse embryonic fibroblast adipose-like cell line

Western Blot

2 µg/mL
Sample: MCF‑7 human breast cancer cell line, Saos‑2 human osteosarcoma cell line, and T47D human breast cancer cell line

Reviewed Applications

Read 2 reviews rated 4.5 using MAB7106 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ER beta/NR3A2

Estrogen Receptor beta (ER beta ; NR3A2) is a member of the steroid receptor family. The natural ligand for ER is the classical estrogenic compound 17 beta -estradiol. ER beta is expressed in the granulosa cell layer of primary, secondary and mature follicles in the ovary, in bone, bladder, uterus, testis, epididymis, gastrointestinal tract, kidney, breast, heart, vessel wall, immune system, lung, pituitary, hippocampus and hypothalamus. Roles for ER beta in the reproductive and cardiovascular systems have been reported, although these are the subject of conflicting reports. ER beta has been postulated to act primarily as a modulator of ER alpha function. ER beta has been shown to form homodimers as well as heterodimers with ER alpha. Both ER alpha and ER beta can give rise to numerous isoforms.

Long Name

Estrogen Receptor beta

Alternate Names

ESR2, NR3A2

Entrez Gene IDs

2100 (Human)

Gene Symbol

ESR2

UniProt

Q92731

Additional ER beta/NR3A2 Products

Product Documents for Human ER beta /NR3A2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ER beta /NR3A2 Antibody

For research use only

Citations for Human ER beta /NR3A2 Antibody

Customer Reviews for Human ER beta /NR3A2 Antibody (2)

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  • Human ER beta /NR3A2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: LNCaP human prostate cancer cell line
    Species: Human
    Verified Customer | Posted 04/11/2022
    Human ER beta /NR3A2 Antibody MAB7106
  • Human ER beta /NR3A2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 human breast cancer cell line and T47D human breast cancer cell line
    Species: Human
    Verified Customer | Posted 07/24/2017

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