Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 91811
View all FGF-18 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived human FGF-18 protein
Glu28-Ala207
Accession # O76093

Specificity

Detects human FGF-18 by direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FGF‑18 Antibody

Detection of FGF‑18 in Human Uterine Cancer.

FGF‑18 was detected in immersion fixed paraffin-embedded sections of Human Uterine Cancer using Mouse Anti-Human FGF‑18 Monoclonal Antibody (Catalog # MAB6671) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasmic staining in tumor stroma. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human FGF‑18 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Uterine Cancer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-18

Fibroblast Growth Factor 18 (FGF-18) is a 20 kDa protein that plays an important role in skeletal development and bone homeostasis (1). Mature human FGF-18 shares 99% amino acid sequence identity with mouse and rat FGF-18 (2). It is expressed in embryonic somites and the neural fold (3), adult lung (2), cerebellar and hippocampal neurons (4), hair follicle root sheath cells (5), and osteogenic mesenchymal cells (6). FGF-18 binds to FGF R2c, FGF R3c (4, 7) as well as the Golgi protein GLG1 (8) and induces the proliferation of astrocytes and microglia, vascular endothelial cells, dermal fibroblasts, papilla cells, and keratinocytes (4, 5, 9). FGF-18 is required for normal skeletal development (6). It recruits osteoclasts and osteoblasts to the growth plate, promotes osteoclast formation and function, inhibits osteoblast differentiation, promotes skeletal vascularization, and induces chondrocyte hypertrophy and cartilage matrix formation (6, 7, 10, 11).

References

  1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
  2. Ohbayashi, N. et al. (1998) J. Biol. Chem. 273:18161.
  3. Ohuchi, H. et al. (2000) Mech. Dev. 95:55.
  4. Hoshikawa, M. et al. (2002) Brain Res. Mol. Brain Res. 105:60.
  5. Kawano, M. et al. (2005) J. Invest. Dermatol. 124:877.
  6. Ohbayashi, N. et al. (2002) Genes Dev. 16:870.
  7. Davidson, D. et al. (2005) J. Biol. Chem. 280:20509.
  8. Miyaoka, Y. et al. (2010) Development 137:159.
  9. Hu, M.C. et al. (1998) Mol. Cell. Biol. 18:6063.
  10. Liu, Z. et al. (2007) Dev. Biol. 302:80.
  11. Shimoaka, T. et al. (2002) J. Biol. Chem. 277:7493.

Long Name

Fibroblast Growth Factor 18

Alternate Names

FGF18

Entrez Gene IDs

8817 (Human)

Gene Symbol

FGF18

UniProt

O76093

Additional FGF-18 Products

Product Documents for Human FGF‑18 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FGF‑18 Antibody

For research use only

Related Research Areas

FGF Family
FGF Ligands

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Protocols

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