Human FoxM1 Antibody [Biotin]

R&D Systems | Catalog # BAF3975

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all FoxM1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxM1
Thr642-Gln763
Accession # Q08050

Specificity

Detects human FoxM1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) FoxJ1 is observed and less than 1% cross-reactivity with rhFoxB2, rhFoxC2, rhFoxD3, rhFoxF1, rhFoxH1, rhFoxO3A, rhFoxP1, and rhFoxP3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human FoxM1 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human FoxM1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FoxM1

FoxM1 (Forkhead box protein M1; also FKHL16, HFH11 and WIN) is a 90‑100 kDa member of the winged helix transcription factor gene family. It is expressed in proliferating and tumor cells and promotes the transition from G1 to S phase and G2 to M phase within the cell cycle. Human FoxM1 is 763 amino acids (aa) in length. It contains three PEST sequences (aa 22‑34, 460‑472 and 478‑515), a forkhead DNA binding domain (aa 235‑327), a Pro/Ser/Thr-rich region (aa 480‑513) and a phosphorylation site at Ser704. There are at least two splice variants. The first shows a 38 aa insertion after Val423. This blocks FoxM1 transcriptional activation activity. The second variant shows a deletion of aa 326‑340. Over aa 642‑763, human FoxM1 shares 73% aa identity with mouse FoxM1.

Long Name

Forkhead Box M1

Alternate Names

FKHL16, HFH-11, HNF-3, INS-1, MPHOSPH2, MPP-2, PIG29, Trident

Entrez Gene IDs

2305 (Human); 14235 (Mouse); 58921 (Rat)

Gene Symbol

FOXM1

UniProt

Q08050

Additional FoxM1 Products

Product Documents for Human FoxM1 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FoxM1 Antibody [Biotin]

For research use only

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