Human GFAP Antibody [Biotin]

R&D Systems | Catalog # BAF2594

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all GFAP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GFAP
Leu292-Met432
Accession # P14136

Specificity

Detects human GFAP in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human GFAP Antibody [Biotin]

GFAP antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

GFAP in Rat Cortical Stem Cells.

GFAP was detected in immersion fixed differentiated rat cortical stem cells using Sheep Anti-Human GFAP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GFAP Antibody [Biotin]

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human astrocytes and immersion fixed rat cortical stem cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human GFAP

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFAP

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament protein. It is the major component of astrocyte intermediate filament. Defects in GFAP are a cause of Alexander disease. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. At the amino acid sequence level, human GFAP shares 91% and 90% identity with rat and mouse GFAP, respectively.

Long Name

Glial Fibrillary Acidic Protein

Alternate Names

ALXDRD, FLJ45472, GFAP, GFAP astrocytes, glial fibrillary acidic protein

Entrez Gene IDs

2670 (Human); 14580 (Mouse); 24387 (Rat)

Gene Symbol

GFAP

UniProt

P14136

Additional GFAP Products

Product Documents for Human GFAP Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GFAP Antibody [Biotin]

For research use only

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Protocols

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