Human ICAM‑1/CD54 Biotinylated Antibody

Name: Human ICAM‑1/CD54 Biotinylated Antibody
Brand: R&D Systems
SKU: BBA9
Price: 499 USD
Availability: InStock
Rating: 4 (1 reviews)

R&D Systems | Catalog # BBA9

(1)

Citations (6)

Product Documents

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Adhesion Blockade, Flow Cytometry, Bioassay, Functional Assay

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # BBIG-I1 (11C81)

View all ICAM-1/CD54 Primary Antibodies »

Product Specifications

Immunogen

Activated human umbilical vein endothelial cells (HUVEC)

Specificity

Detects human ICAM-1 in Western blots. In this format, this antibody does not cross‑react with rhCEACAM-1, rhICAM-2, -3, -5, rmICAM-2, rrICAM-1, rmMAdCAM, or rhVCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human ICAM‑1/CD54 Biotinylated Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human ICAM‑1/CD54 Fc Chimera (Catalog # 720-IC)

Reviewed Applications

Read 1 review rated 4 using BBA9 in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: ICAM-1/CD54

ICAM-1 is a member of the immunoglobulin superfamily whose expression is upregulated on leukocytes, epithelial cells, and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.