Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, ELISA, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1000011
View all Ig Lambda Light Chain Primary Antibodies »

Product Specifications

Immunogen

Ig Lambda Light Chain

Specificity

Detects Human Ig Lambda Light Chain in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Ig Lambda Light Chain Antibody

Detection of IgLambda in Human Tonsil via seqIF™ staining on COMET™

IgLambda was detected in immersion fixed paraffin-embedded sections of human Tonsil using Mouse Anti-Human IgLambda, Monoclonal Antibody (Catalog # MAB10049) at 0.5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of Human IgG Lambda Light Chain antibody by Western Blot.

Detection of Human IgG Lambda Light Chain by Western Blot.

Western blot shows human kappa light chain protein, human lambda light chain protein, human serum, primate serum, mouse serum, and rat serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB10049) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ig Lambda Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Ig Lambda Light Chain antibody by Simple WesternTM.

Detection of Human Ig Lambda Light Chain by Simple WesternTM.

Simple Western lane view shows lysates of human kappa light chain protein (negative control), human lambda light chain protein, and human serum, loaded at 0.2 mg/mL. A specific band was detected for Ig Lambda Light Chain at approximately 35 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB10049). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Human Ig Lambda Light Chain Antibody in ELISA Standard Curve.

Human Ig Lambda Light Chain ELISA Standard Curve.

Human Ig Lambda Light Chain protein was serially diluted 2-fold and captured by Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB100491) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB10049) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Detection of Ig Lambda Light Chain in Human Tonsil.

Ig Lambda Light Chain was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB10049) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Ig Lambda Light Chain Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB100491).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Multiplex Immunofluorescence

0.5 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Tonsil

Simple Western

20 µg/mL
Sample: Human Lambda Light Chain and Human serum

Western Blot

2 µg/mL
Sample: Human Lambda Light Chain and Human serum

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ig Lambda Light Chain

The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa ( kappa ) or a lambda ( lambda ) chain.  Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.

Long Name

Immunoglobulin Lambda Light Chain

Alternate Names

Bence Jones protein, BJP, Constant region of lambda light chains, HCAK 1, HCAK1, Ig kappa chain C region, Ig lambda chain C regions, IGKC, IGLC, IGLC 1, IGLC 2, IGLC 3, IGLC1, IGLC2, IGLC3, Immunoglobulin kappa constant, Immunoglobulin kappa constant region, Immunoglobulin kappa light chain, Immunoglobulin lambda constant 1, Kappa 1 immunoglobulin light chain, Km, Mcg marker, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809, Paraprotein

Additional Ig Lambda Light Chain Products

Product Documents for Human Ig Lambda Light Chain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Ig Lambda Light Chain Antibody

For research use only

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Protocols

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