Human IL-8/CXCL8 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC208P
Human IL-8/CXCL8 PE-conjugated Antibody in Data
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Product Details
Citations (4)
FAQs
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Reviews (1)

Human IL-8/CXCL8 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL8/IL‑8 in ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant porcine CXCL8/IL‑8 and no cross-reactivity with rrCXCL3/CINC-2 alpha.
Source
Monoclonal Mouse IgG1 Clone # 6217
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CXCL8/IL‑8
Ser28-Ser99
Accession # P10145
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of CXCL8/IL‑8 in Human Blood Monocytes by Flow Cytometry. View Larger

Detection of CXCL8/IL‑8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL8/IL-8 PE-conjugated Monoclonal Antibody (Catalog # IC208P) and Anti-Human CD14 Fluorescein-conjugated Antibody (panel A). Inhibition of IC208P staining by the addition of excess Recombinant Human IL-8 (Catalog # 208-IL) is shown in panel B. Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-8/CXCL8

CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.

Long Name
Interleukin 8
Entrez Gene IDs
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

Product Datasheets

Citations for Human IL-8/CXCL8 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. TGFb1 suppresses the activation of distinct dNK subpopulations in preeclampsia
    Authors: J Zhang, CE Dunk, O Shynlova, I Caniggia, SJ Lye
    EBioMedicine, 2019;39(0):531-539.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Downregulation of Pro-Inflammatory and Pro-Angiogenic Pathways in Prostate Cancer Cells by a Polyphenol-Rich Extract from Olive Mill Wastewater
    Authors: D Baci, M Gallazzi, C Cascini, M Tramacere, D De Stefano, A Bruno, DM Noonan, A Albini
    Int J Mol Sci, 2019;20(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Gene and protein characteristics reflect functional diversity of CD56dim and CD56bright NK cells.
    Authors: Wendt K, Wilk E, Buyny S, Buer J, Schmidt RE, Jacobs R
    J. Leukoc. Biol., 2006;80(6):1529-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
    Authors: Epelman S, Stack D, Bell C, Wong E, Neely GG, Krutzik S, Miyake K, Kubes P, Zbytnuik LD, Ma LL, Xie X, Woods DE, Mody CH
    J. Immunol., 2004;173(3):2031-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human IL-8/CXCL8 PE-conjugated Antibody

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Human IL-8/CXCL8 PE-conjugated Antibody
By Anonymous on 04/03/2018
Application: Flow Sample Tested: Serum Species: Human