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Human Integrin  beta 6 Antibody

Catalog #: MAB4155
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4155
MAB4155-SP

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Detection of Integrin beta 6 in HT‑29 Human Cell Line by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  beta 6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 6 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 437211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha V beta 6
Phe31-Val992 (Integrin alpha V) and Gly22-Asn707 (Integrin beta 6)
Accession # P18564
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin beta 6 antibody in HT‑29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin beta 6 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Human Integrin beta 6 Monoclonal Antibody (Catalog # MAB4155, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Immunocytochemistry Integrin beta 6 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin beta 6 in HT‑29 Human Cell Line. Integrin beta 6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human Integrin beta 6 Monoclonal Antibody (Catalog # MAB4155) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

Detection of Integrin  beta 6 in HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells. Integrin  beta 6 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive) and A549 human lung carcinoma cell line (negative) cells using Mouse Anti-Human Integrin  beta 6 Monoclonal Antibody (Catalog # MAB4155) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 6

Integrin beta 6 (ITG beta 6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha V and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta 6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta 6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta 6 ECD, respectively.

Entrez Gene IDs
3694 (Human); 16420 (Mouse); 311061 (Rat); 102122296 (Cynomolgus Monkey)
Alternate Names
Integrin beta 6; integrin beta-6; integrin, beta 6; ITGB6

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Citations for Human Integrin  beta 6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Spatial Distribution of Non-Immune Cells Expressing Glycoprotein A Repetitions Predominant in Human and Murine Metastatic Lymph Nodes
    Authors: Rouaud, L;Baudin, L;Gautier-Isola, M;Van Meerbeeck, P;Feyereisen, E;Blacher, S;van Baren, N;Kridelka, F;Lucas, S;Noel, A;
    Cancers
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Integrin-alphaV-mediated activation of TGF-beta regulates anti-tumour CD8 T cell immunity and response to PD-1 blockade
    Authors: I Malenica, J Adam, S Corgnac, L Mezquita, E Auclin, I Damei, L Grynszpan, G Gros, V de Montpré, D Planchard, N Théret, B Besse, F Mami-Choua
    Nature Communications, 2021-09-01;12(1):5209.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2010-10-07;134(2):167-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Cryo-EM Reveals Integrin-Mediated TGF-beta Activation without Release from Latent TGF-beta
    Authors: Melody G. Campbell, Anthony Cormier, Saburo Ito, Robert I. Seed, Andrew J. Bondesson, Jianlong Lou et al.
    Cell
  5. CBP/beta-Catenin/FOXM1 Is a Novel Therapeutic Target in Triple Negative Breast Cancer
    Authors: Ring A, Nguyen C, Smbatyan G et al.
    Cancers (Basel).

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