Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Dual RNAscope ISH-IHC Compatible

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Cell-based ELISA, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 36006
View all MMP-2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human MMP-2

Specificity

Detects pro/active forms of human MMP-2 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP‑9, rhMMP-1, or rhMMP-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MMP-2 Antibody (36006)

MMP‑2 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

MMP‑2 in Human Ovarian Cancer Tissue.

MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human MMP-2 Monoclonal Antibody (Catalog # MAB902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MMP‑2 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

MMP‑2 in Human Ovarian Cancer Tissue.

MMP‑2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human MMP‑2 Monoclonal Antibody (Catalog # MAB902) at 15 µg/mL overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of MMP‑2 in Human Stomach.

Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for MMP2 mRNA (ACD RNAScope Probe, catalog #311751; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human MMP2 monoclonal antibody (R&D Systems catalog # MAB902) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to fibroblasts.
Detection of MMP-2 by Immunocytochemistry/ Immunofluorescence

Detection of MMP-2 by Immunocytochemistry/ Immunofluorescence

Treatment with IL‐17RA or IL‐17RC neutralizing antibodies reduces MMP‐2 and MMP‐9 expression and restores the fibrinolytic system in BLM‐induced lung fibrosis: (A) Representative blots and densiometric analysis of MMP‐2 and MMP‐9 21 days post‐BLM administration. beta ‐Actin was used as a loading control. The immunofluorescence images with respective graphs of (b) MMP‐2 at 21 days post‐BLM administration, (c) MMP‐9 at 21 days post‐BLM treatment, Scale bar‐25 μm. Data were analyzed using One‐way ANOVA and Sidak's multiple comparison test. Data are shown as mean ± SD. Full‐length blots with molecular weight markers are provided in the Figure S1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/42298666), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of MMP-2 by Immunocytochemistry/ Immunofluorescence

Detection of MMP-2 by Immunocytochemistry/ Immunofluorescence

Treatment with IL‐17RA or IL‐17RC neutralizing antibodies reduces MMP‐2 and MMP‐9 expression and restores fibrinolytic system in BLM‐induced lung fibrosis: (A) Representative blots and densiometric analysis of MMP‐2 at 14th day post‐BLM administration, beta ‐Actin was used as loading control. The immunofluorescence images with representative graphs of (b) MMP‐2 at 14 days post‐BLM administration, (c) MMP‐9 at 14 days post‐BLM treatment, Scale bar‐25 μm. Data were analyzed using One‐way ANOVA and Sidak's multiple comparison test. Data are shown as mean ± SD. Full‐length blots with molecular weight markers are provided in the Figure S1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/42298666), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of MMP-2 by Western Blot

Detection of MMP-2 by Western Blot

Differential regulation of MMP‐2 and uPA by IL‐17A receptors: (a) Representative western blot image of uPA and MMP‐2, (b) densiometric analysis of uPA and (c) densiometric analysis of MMP‐2 14 days post‐BLM administration, (d) Representative western blot image of MMP‐2, (b) densiometric analysis of MMP‐2 21 days post‐BLM administration. Data were analyzed using One‐way ANOVA and Sidak's multiple comparison test. Data are shown as mean ± SD. Full‐length blots with molecular weight markers are provided in the Figure S1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/42298666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MMP-2 Antibody (36006)

Application
Recommended Usage

Dual RNAscope ISH-IHC Compatible

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Immunohistochemistry

50-100 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue

Western Blot

1 µg/mL
Sample: Recombinant Human MMP‑2 Western Blot Standard (Catalog # WBC025) under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting;and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Gene Symbol

MMP2

Additional MMP-2 Products

Product Documents for Human MMP-2 Antibody (36006)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP-2 Antibody (36006)

For research use only

Citations for Human MMP-2 Antibody (36006)

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Protocols

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