Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 603813
View all ATG5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ATG5
Asn99-Thr193
Accession # Q9H1Y0

Specificity

Detects human, mouse, and rat ATG5 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat ATG5 Antibody

Detection of Human, Mouse, and Rat ATG5 antibody by Western Blot.

Detection of Human, Mouse, and Rat ATG5 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CH-1 mouse B cell lymphoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat ATG5 Monoclonal Antibody (Catalog # MAB5294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATG5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human and Rat ATG5 antibody by Simple WesternTM.

Detection of Human and Rat ATG5 by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG5 at approximately 51 kDa (as indicated) using 25 µg/mL of Mouse Anti-Human/Mouse/Rat ATG5 Monoclonal Antibody (Catalog # MAB5294). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat ATG5 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded human small intestine

Simple Western

25 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑12 rat adrenal pheochromocytoma cell line

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, CH‑1 mouse B cell lymphoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB5294 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ATG5

ATG5 (Autophagy-related Protein), also known as APG5L and Apoptosis-specific Protein, is a ubiquitous 32 kDa member of the ATG family of proteins. ATG5 exists as a covalent heterodimer with ATG12 through the creation of a Lys-Gly linkage. The ATG5:ATG12 heterodimer associates noncovalently with an ATG16 multimer to generate autophagosomes. Human ATG5 is 275 amino acids in length and contains N- and C-terminal ubiquitin-like domains (aa 15‑105 and 187‑275) separated by a helix-rich linker region that contains a dimerizing Lys at position 130. There are two potential alternate start sites at Met80 and Met173. Over aa 99‑193, human ATG5 is 97% aa identical to mouse ATG5.

Long Name

ATG5 Autophagy Related 5 Homolog

Alternate Names

APG5, ASP

Entrez Gene IDs

9474 (Human); 11793 (Mouse)

Gene Symbol

ATG5

UniProt

Q9H1Y0 (Human)

Additional ATG5 Products

Product Documents for Human/Mouse/Rat ATG5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat ATG5 Antibody

For research use only

Related Research Areas

Autophagy

Citations for Human/Mouse/Rat ATG5 Antibody

Customer Reviews for Human/Mouse/Rat ATG5 Antibody (1)

5 out of 5
1 Customer Rating
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1 Stars
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  • Human/Mouse/Rat ATG5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: CH-1 mouse B cell lymphoma cell line
    Species: Mouse
    Verified Customer | Posted 07/25/2022
    Human/Mouse/Rat ATG5 Antibody MAB5294

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Protocols

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