Human/Mouse/Rat CDK2 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF4654V Datasheet / COA / SDS

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AF4654V-100UG

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Human/Mouse/Rat CDK2 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat CDK2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) CDK4 and rhCDK6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CDK2
Met1-Leu298
Accession # P24941

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CDK2

CDK2 (cyclin-dependent kinase 2), a serine/threonine protein kinase, is the catalytic subunit of the heterodimeric cyclin-dependent kinase complex required for G1/S phase transition. The kinase activity of CDK2 is regulated by the association with a cyclin subunit, CDK inhibitors, and its phosphorylation state. The active CDK2/cyclin A complex interacts with the N-terminus of E2F-1 and directs the phosphorylation of E2F-1 and DP-1. The active CDK2/cyclin E complex phosphorylates Rb which disrupts its binding to E2F, allowing E2F activation and transcription of the genes necessary for S-phase entry and progression. Inhibition of the CDK2/cyclin complex can be attributed to its association with p21^Waf1/Cip1 and p27^Kip1 and the phosphorylation of CDK2 on Thr14 and Tyr15. The activation of the CDK2/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Tyr14 and Tyr15.

Long Name

Cyclin-dependent Kinase 2

Entrez Gene IDs

1017 (Human); 12566 (Mouse); 362817 (Rat)

Alternate Names

cdc2-related protein kinase; CDK2; cell devision kinase 2; Cell division protein kinase 2; cyclin-dependent kinase 2; EC 2.7.11; EC 2.7.11.22; p33 protein kinase; p33(CDK2)