Human/Mouse/Rat p27/Kip1 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB22561S Datasheet / COA / SDS

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FAB22561S-100UG

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All p27/Kip1 Antibodies

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Human/Mouse/Rat p27/Kip1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human p27/Kip1 in direct ELISAs. Specificity was demonstrated by comparing staining on tissue sections of normal versus p27/Kip1 knockout mice.

Source

Monoclonal Rat IgG2a Clone # 341116

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human p27/Kip1
Met1-Thr198
Accession # P46527

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: p27/Kip1

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. Human p27/Kip1 shares 88% and 87% amino acid sequence identity with mouse and rat p27/Kip1, respectively, while mouse and rat p27/Kip1 share 96% amino acid sequence identity.

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Entrez Gene IDs

1027 (Human); 12576 (Mouse); 83571 (Rat)

Alternate Names

CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1