UCH-L1/PGP9.5 Antibody (972119)

R&D Systems | Catalog # MAB60072

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 972119
View all UCH-L1/PGP9.5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human UCH-L1/PGP9.5
Gln2-Ala223
Accession # P09936

Specificity

Detects human UCH-L1/PGP9.5 in direct ELISAs. Detects human, mouse, and rat in Western blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for UCH-L1/PGP9.5 Antibody (972119)

Detection of Human, Mouse, and Rat UCH-L1/PGP9.5 antibody by Western Blot.

Detection of Human, Mouse, and Rat UCH-L1/PGP9.5 by Western Blot.

Western blot shows lysates of A172 human glioblastoma cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB60072) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for UCH-L1/PGP9.5 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
UCH-L1/PGP9 by Immunocytochemistry (ICC).5 antibody in A172 Human Cell Line by Immunocytochemistry (ICC).

UCH-L1/PGP9.5 in A172 Human Cell Line.

UCH-L1/PGP9.5 was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB60072) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
UCH-L1/PGP9 by Immunohistochemistry (IHC-P).5 antibody in Human Brain by Immunohistochemistry (IHC-P).

UCH-L1/PGP9.5 in Human Brain.

UCH-L1/PGP9.5 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB60072) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human and Mouse UCH-L1/PGP9.5 antibody by Simple WesternTM.

Detection of Human and Mouse UCH-L1/PGP9.5 by Simple WesternTM.

Simple Western lane view shows lysates of A172 human glioblastoma cell line and Neuro‑2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for UCH-L1/PGP9.5 at approximately 31 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB60072). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
UCH-L1/PGP9.5 in Human Colon.

UCH-L1/PGP9.5 in Human Colon.

1:100 dilution in PFA-fixed, frozen human sigmoid colon. 0.2% Triton X-100 was included in primary antibody mix. Image from a verified customer review.

Applications for UCH-L1/PGP9.5 Antibody (972119)

Application
Recommended Usage

Immunocytochemistry

0.3-25 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus)

Simple Western

1 µg/mL
Sample: A172 human glioblastoma cell line and Neuro‑2A mouse neuroblastoma cell line

Western Blot

0.1 µg/mL
Sample: A172 human glioblastoma cell line, Neuro‑2A mouse neuroblastoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UCH-L1/PGP9.5

UCH-L1 (ubiquitin carboxyterminal hydrolase isozyme 1; also PGP9.5) is a 24-27 kDa member of the peptidase C12 family of enzymes. It shows restricted expression, being found in neurons and oocytes. UCH-L1 has dual enzymatic activity. As a monomer, it is a ubiquitin hydrolase that removes ubiquitin from modified proteins; as a homodimer, it acts as a ligase that creates ubiquitin dimers. In neurons, UCH-L1’s most important role appears to be that of generating free ubiquitin. Human UCH-L1 is 223 amino acids (aa) in length. It is O-glycosylated, ubiquitinated, and farnesylated; when farnesylated, it becomes associated with cell membranes. Three potential splice forms are reported. One shows a two aa substitution for aa 12-15, a second contains an alternative start site at Met82, and a third shows the same start site coupled with a deletion of aa 138-153. Full-length human UCH-L1 shares 95% aa identity with mouse UCH-L1.       

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Entrez Gene IDs

7345 (Human); 22223 (Mouse)

Gene Symbol

UCHL1

UniProt

P09936 (Human)

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody (972119)

Certificate of Analysis

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Product Specific Notices for UCH-L1/PGP9.5 Antibody (972119)

For research use only

Citations for UCH-L1/PGP9.5 Antibody (972119)

Customer Reviews for UCH-L1/PGP9.5 Antibody (972119) (1)

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  • Name: Tom Leech
    Verified Customer | Posted 10/16/2025
    1:100 dilution shows strong staining in human myenteric plexus
    1:100 dilution in PFA-fixed, frozen human sigmoid colon. 0.2% Triton X-100 was included in primary antibody mix.
    UCH-L1/PGP9.5 Antibody (972119) MAB60072

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