Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 779427
View all Tyrosine Hydroxylase Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Tyrosine Hydroxylase
Ala278-Tyr401
Accession # P07101

Specificity

Detects human Tyrosine Hydroxylase in direct ELISAs and human, mouse and rat Tyrosine Hydroxylase in Western blots. In direct ELISAs, no cross-reactivity with recombinant human Tryptophan Hydroxylase-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse Tyrosine Hydroxylase Antibody

Detection of Human, Mouse, and Rat Tyrosine Hydroxylase antibody by Western Blot.

Detection of Human, Mouse, and Rat Tyrosine Hydroxylase by Western Blot.

Western blot shows lysates of human adrenal gland tissue, mouse adrenal gland tissue, and rat adrenal gland tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Tyrosine Hydroxylase at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Tyrosine Hydroxylase antibody in Mouse Dopaminergic Neurons by Immunocytochemistry (ICC).

Tyrosine Hydroxylase in Mouse Dopaminergic Neurons.

Tyrosine Hydroxylase was detected in immersion fixed mouse embryonic stem cells differentiated into dopaminergic neurons using Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). Cells were double stained using the Northern-Lights™ 637-conjugated Mouse Anti-Neuron-specific beta-III Tubulin Monoclonal Antibody (white; Catalog # NL1195V). Cells were counterstained with DAPI (blue). Specific staining of Tyrosine Hydroxylase was localized to cytoplasm of dopaminergic neurons. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Tyrosine Hydroxylase antibody in Human Brain by Immunohistochemistry (IHC-P).

Tyrosine Hydroxylase in Human Brain.

Tyrosine Hydroxylase was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human and Mouse Tyrosine Hydroxylase antibody by Simple WesternTM.

Detection of Human and Mouse Tyrosine Hydroxylase by Simple WesternTM.

Simple Western lane view shows lysates of human adrenal gland tissue and mouse adrenal gland tissue, loaded at 0.2 mg/mL. Specific bands were detected for Tyrosine Hydroxylase at approximately 52-61 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human/Mouse Tyrosine Hydroxylase Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse embryonic stem cells differentiated into dopaminergic neurons

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla)

Simple Western

5 µg/mL
Sample: human adrenal gland tissue and mouse adrenal gland tissue

Western Blot

0.25 µg/mL
Sample: Human adrenal gland tissue, mouse adrenal gland tissue, and rat adrenal gland tissue

Reviewed Applications

Read 4 reviews rated 4.8 using MAB7566 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tyrosine Hydroxylase

TH (Tyrosine 3-hydroxylase), also known as Tyrosine 3-monooxygenase, is a 60-62 kDa member of the biopterin-dependent aromatic amino acid hydroxylase family of molecules. It is expressed by neurons of the dopamine and autonomic nervous system, plus the neuroendocrine cells of the adrenal medulla. TH is considered the rate limiting enzyme for catecholamine synthesis, and serves to catalyze the hydroxylation of L-tyrosine. It maintains stores of catecholamines following secretion, and its activity is regulated by targeted site phosphorylation. Human TH is 528 amino acids (aa) in length. It contains an N-terminal ACT domain (aa 69-190) that binds small molecules and regulates enzyme activity, and a C-terminal enzymatic region (aa 196-493). There are three significant utilized phosphorylation sites. Two at Ser31 and Ser40 increase enzyme activity, while phosphorylation at Ser19 promotes subsequent Ser40 phosphorylation. TH functions as a 240 kDa noncovalent homotetramer. There are four potential splice variants. One shows a deletion of aa 31-34, a second shows a deletion of aa 31-61, while a third contains a Met substitution for aa 1-34. A fourth isoform variant shows a deletion of aa 35-61. Over aa 278-401, human TH shares 94% aa sequence identity with mouse TH, a molecule that most closely resembles the fourth human isoform variant described above.

Alternate Names

TH, TYH

Entrez Gene IDs

7054 (Human); 21823 (Mouse); 25085 (Rat)

Gene Symbol

TH

UniProt

P07101

Additional Tyrosine Hydroxylase Products

Product Documents for Human/Mouse Tyrosine Hydroxylase Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Tyrosine Hydroxylase Antibody

For research use only

Citations for Human/Mouse Tyrosine Hydroxylase Antibody

Customer Reviews for Human/Mouse Tyrosine Hydroxylase Antibody (4)

4.8 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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Customer Images

Showing  1 - 4 的 4 reviews Showing All
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  • Human/Mouse Tyrosine Hydroxylase Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 09/13/2021
    Human/Mouse Tyrosine Hydroxylase Antibody MAB7566
  • Human/Mouse Tyrosine Hydroxylase Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Sympathetic nerve and conjunctival sympathetic nerve
    Species: Mouse
    Verified Customer | Posted 10/27/2017
    Human/Mouse Tyrosine Hydroxylase Antibody MAB7566
  • Human/Mouse Tyrosine Hydroxylase Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 10/24/2016
    TH positive neurons in red, co-stained with TUJ1 to mark neurons.
    Human/Mouse Tyrosine Hydroxylase Antibody MAB7566
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen tissue sections or rat cerebrum
    Species: Rat
    Verified Customer | Posted 09/02/2015
    TH positive cells, IHC-Fr 1:500 dilution
    Human/Mouse Tyrosine Hydroxylase Antibody MAB7566

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Protocols

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