Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1006811
View all MT2A Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human MT2A
Accession # P02795

Specificity

Detects human MT2A in diect ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human MT2A Antibody

MT2A antibody in Human Liver by Immunohistochemistry (IHC-P).

MT2A in Human Liver.

MT2A was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human MT2A Monoclonal Antibody (Catalog # MAB10176) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human MT2A Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Reviewed Applications

Read 1 review rated 5 using MAB10176 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MT2A

Metallothionein-2 (MT2A) is a protein that is transcriptionally regulated by heavy metals and glucocorticoids. This Metallothionein (MT) binds zinc. MTs sequester and/or dispense zinc during stress and inflammation to protect cells against reactive oxygen species.

Long Name

Metallothionein 2A

Alternate Names

MT-II

Entrez Gene IDs

4502 (Human); 17750 (Mouse)

Gene Symbol

MT2A

UniProt

P02795

Additional MT2A Products

Product Documents for Human MT2A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MT2A Antibody

For research use only

Citations for Human MT2A Antibody

Customer Reviews for Human MT2A Antibody (1)

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  • Human MT2A Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver tissue
    Species: Human
    Verified Customer | Posted 06/05/2022
    Human MT2A Antibody MAB10176

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Protocols

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