Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 259416
View all NKX2.5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NKX2.5
Gln24-Arg137
Accession # P52952

Specificity

Detects human NKX-2.5 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NKX2.5 Antibody

NKX2 by Immunohistochemistry (IHC-P).5 antibody in Human Heart by Immunohistochemistry (IHC-P).

NKX2.5 in Human Heart.

NKX2.5 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human NKX2.5 Monoclonal Antibody (Catalog # MAB2444) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human NKX2.5 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart and perfusion fixed frozen sections of rat heart

Reviewed Applications

Read 1 review rated 4 using MAB2444 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKX2.5

NKX2.5, also called NKX-2E or Csx (Cardiac specific homeobox protein), is a transcriptional activator. Its expression is essential for normal cardiovascular development. During embryonic development, NKX2.5 is also expressed in the foregut, thyroid, spleen, and stomach, while in the adult expression is predominantly restricted to the heart.

Long Name

NK2 Homeobox 5

Alternate Names

CSX1, NKX2-5, NKX2E

Entrez Gene IDs

1482 (Human)

Gene Symbol

NKX2-5

UniProt

P52952

Additional NKX2.5 Products

Product Documents for Human NKX2.5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NKX2.5 Antibody

For research use only

Citations for Human NKX2.5 Antibody

Customer Reviews for Human NKX2.5 Antibody (1)

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  • Human NKX2.5 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Embryonic stem cells differentiated into dopaminergic neurons
    Species: Human
    Verified Customer | Posted 01/16/2018

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Protocols

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