Human Periostin/OSF-2 Antibody

R&D Systems | Catalog # AF3548

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Periostin/OSF-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Periostin
Asn22-Gln836
Accession # Q15063

Specificity

Detects human Periostin/OSF-2 in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross‑reactivity with recombinant mouse Periostin and recombinant rat Periostin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Periostin/OSF-2 Antibody

Immunoprecipitation of Human Periostin/OSF-2.

Immunoprecipitation of Human Periostin/OSF-2.

Human Periostin/OSF-2 was immunoprecipitated from human milk samples diluted in 1X Sample Diluent Concentrate 2 (DYC002) and incubated with 3 µg Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) or Normal Goat IgG Control (AB-108-C) plus 30 µL Protein G beads overnight. Immunoprecipitated Periostin/OSF-2 was detected by Western blot under reducing conditions using 1 µg/mL Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548). View our recommended buffer recipes for immunoprecipitation.
Periostin/OSF-2 antibody in Human Breast by Immunohistochemistry (IHC-P).

Periostin/OSF‑2 in Human Breast.

Periostin/OSF-2 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Periostin/OSF‑2 by Western Blot.

Western blot shows lysates of Human Breast Cancer. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Periostin/OSF‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Periostin/OSF‑2 at approximately ~75kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Periostin/OSF-2 by Immunohistochemistry

Detection of Periostin/OSF-2 by Immunohistochemistry

Representative immunohistochemical staining of low and high expressions of tumour-based.(A) E-cad, (B) beta -cat, (C) Fas, (D) Snail, (E) Zeb-1, and (F) stromal PN in CRC tissues. The scale bar represents 100 μm. Original magnification of 200X. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38935747), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Periostin/OSF-2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast

Immunoprecipitation

25 µg/mL
Sample: Human milk

Western Blot

1 µg/mL
Sample: Human breast cancer tissue

Reviewed Applications

Read 1 review rated 5 using AF3548 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Periostin/OSF-2

Human OSF-2 (Osteoblast-Specific Factor 2), also known as Periostin, is a 170 kDa secreted homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules. It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor. It is synthesized as an 836 amino acid (aa) precursor that contains a 21 aa signal sequence and an 815 aa mature region. It is unknown if the molecule has any significant glycosylation. The human homodimer is not disulfide-linked. The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four 130 aa Fasciculin type 1 (FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix. Each FAS1 repeat exhibits a novel seven-stranded beta -wedge with a multiple alpha -helical fold. Three alternate splice forms are known that are C-terminal to the fourfold FAS1 repeats. These mature molecules are 758 and 761 aa in length. The first shows a one aa substitution for aa 649-706 of the mature molecule. The second shows a one aa substitution for aa 649-676, and a deletion of 27 aa between aa 784-810 of the mature molecule. The significance of the alternate splice forms is not clear. OSF-2 is known to bind to alpha v beta 3 and alpha v beta 5 integrins. It is synthesized by smooth muscle cells, fibroblasts, osteoblasts, and multiple carcinoma cell types. OSF-2 induces expression of VEGFR2/KDR on endothelial cells (EC) by binding to EC alpha v beta 3. It also promotes cell transformation to a tumorigenic phenotype, accompanied by MMP-9 and fibronectin production and cell migration. Mature human OSF-2 is 91%, 96% and 91% aa identical to rat, dog, and mouse OSF-2, respectively.

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Entrez Gene IDs

10631 (Human); 50706 (Mouse); 361945 (Rat)

Gene Symbol

POSTN

UniProt

Q15063

Additional Periostin/OSF-2 Products

Product Documents for Human Periostin/OSF-2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Periostin/OSF-2 Antibody

For research use only

Citations for Human Periostin/OSF-2 Antibody

Customer Reviews for Human Periostin/OSF-2 Antibody (1)

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  • Human Periostin/OSF-2 Antibody
    Name: Mohamad Youness
    Application: Immunocytochemistry
    Sample Tested: primary human cardiac fibroblasts
    Species: Human
    Verified Customer | Posted 08/01/2023
    Primary human cardiac fibroblasts stained with POSTN (green, 1:100) and DAPI (blue)
    Human Periostin/OSF-2 Antibody AF3548
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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