Human phospho-c-Jun (S63) Antibody (1018Y)

R&D Systems | Catalog # MAB8930

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1018Y
View all c-Jun Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human c-Jun S63 site
Accession # P05412

Specificity

Detects human c-Jun when phosphorylated at S63 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human phospho-c-Jun (S63) Antibody (1018Y)

Detection of Human Phospho-c-Jun (S63) antibody by Western Blot.

Detection of Human Phospho-c‑Jun (S63) by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-c-Jun (S63) Monoclonal Antibody (Catalog # MAB8930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-c-Jun (S63) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
c-Jun antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

c‑Jun in HeLa Human Cell Line.

c-Jun phosphorylated at S63 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with Anisomycin using Rabbit Anti-Human Phospho-c-Jun (S63) Monoclonal Antibody (Catalog # MAB8930) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Phospho-c-Jun (S63) antibody by Simple WesternTM.

Detection of Human Phospho-c‑Jun (S63) by Simple WesternTM.

Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) with a 30 minute recovery, loaded at 0.2 mg/mL. A specific band was detected for Phospho-c‑Jun (S63) at approximately 49 kDa (as indicated) using 5 μg/mL of Rabbit Anti-Human Phospho-c‑Jun (S63) Monoclonal Antibody (Catalog # MAB8930). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human phospho-c-Jun (S63) Antibody (1018Y)

Application
Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line treated with Anisomycin

Simple Western

5 µg/mL
Sample: HEK293T human embryonic kidney cell line treated with ultraviolet light (UV)

Western Blot

0.5 µg/mL
Sample: HEK293T human embryonic kidney cell line treated with ultraviolet light (UV)

Reviewed Applications

Read 2 reviews rated 5 using MAB8930 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: c-Jun

c-Jun is one of three Jun family proteins making up the Activator Protein-1 (AP-1) transcription factor group. c-Jun acts as a positive regulator of cell proliferation and can induce oncogenic transformation.

Long Name

Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1

Alternate Names

cJun, JUN

Entrez Gene IDs

3725 (Human)

Gene Symbol

JUN

UniProt

P05412

Additional c-Jun Products

Product Documents for Human phospho-c-Jun (S63) Antibody (1018Y)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human phospho-c-Jun (S63) Antibody (1018Y)

For research use only

Citations for Human phospho-c-Jun (S63) Antibody (1018Y)

Customer Reviews for Human phospho-c-Jun (S63) Antibody (1018Y) (2)

5 out of 5
2 Customer Ratings
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  • Human Phospho-c-Jun (S63) Antibody
    Name: Felix Oppel
    Application: Indirect Immunofluorescence
    Sample Tested: Cell culture
    Species: Human
    Verified Customer | Posted 05/03/2022
    Fetuin B antibody in indirect immunofluorescence staining of human tumor cells using a red-fluorescent secondary antibody. Nuclei stained with DAPI (blue). p-c-Jun was stained simultaneously using green-fluorescent secondary antibody.
    Human phospho-c-Jun (S63) Antibody (1018Y) MAB8930
  • Human Phospho-c-Jun (S63) Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: BT-20 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 05/22/2019
    Human phospho-c-Jun (S63) Antibody (1018Y) MAB8930

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Protocols

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