Human phospho-EGFR (Y1068) Antibody (338324)

R&D Systems | Catalog # MAB3570

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 338324
View all EGFR Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human EGFR Y1068 site

Specificity

Detects human EGFR phosphorylated at Y1068.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human phospho-EGFR (Y1068) Antibody (338324)

Detection of Human Phospho-EGFR (Y1068) antibody by Western Blot.

Detection of Human Phospho-EGFR (Y1068) by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho-EGFR (Y1068) Monoclonal Antibody (Catalog # MAB3570), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-EGFR (Y1068) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Phospho-EGFR antibody in EGF-treated A431 Human Cell Line antibody by Flow Cytometry.

Detection of Phospho-EGFR in EGF-treated A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line was untreated (open histogram) or treated for 5 minutes with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG, filled histogram) then stained with Human Phospho--EGFR (Y1068) Monoclonal Antibody (Catalog # MAB3570), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). Mouse IgG2A(Catalog # MAB003, data not shown) was used as an isotype control. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human phospho-EGFR (Y1068) Antibody (338324)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG), fixed with paraformaldehyde, and permeabilized with saponin

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB3570 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGFR

Epidermal growth factor receptor (EGFR, also known as ErbB1 and HER1) is the founding member of the ErbB family of receptor tyrosine kinases. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation at Tyr 1068 allows binding of the SH2 domain of the cytosolic adaptor Grb2. This binding results in Ras activation.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

Additional EGFR Products

Product Documents for Human phospho-EGFR (Y1068) Antibody (338324)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human phospho-EGFR (Y1068) Antibody (338324)

For research use only

Citations for Human phospho-EGFR (Y1068) Antibody (338324)

Customer Reviews for Human phospho-EGFR (Y1068) Antibody (338324) (2)

4.5 out of 5
2 Customer Ratings
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  • Human Phospho-EGFR (Y1068) Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human phospho-EGFR (Y1068) Antibody (338324) MAB3570
  • Human Phospho-EGF R/ErbB1 (Y1068) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: EGF-Stimulated A431 Cell lysate; EGF-Stimulated rat neurons
    Species: Human
    Verified Customer | Posted 10/18/2016
    A: EGF-Stimulated A431 Cell lysate, 20 μg; B: EGF-Stimulated rat neurons, 20 μg. MAB3570 has a high specificity for human phosphorylated EGFR, being unable to identify the rat isoform.
    Human phospho-EGFR (Y1068) Antibody (338324) MAB3570

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