Human phospho-p38 alpha (T180/Y182) Antibody (948243)

Name: Human phospho-p38 alpha (T180/Y182) Antibody (948243)
Brand: R&D Systems
SKU: MAB8692
Price: 459 USD
Availability: InStock

R&D Systems | Catalog # MAB8692

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Rat

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2B Clone # 948243

View all p38 alpha Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human p38 alpha T180/Y182 site
Accession # Q16539

Specificity

Detects human p38 alpha when phosphorylated at T180/Y182.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Human phospho-p38 alpha (T180/Y182) Antibody (948243)

Phospho-p38 alpha (T180/Y182) in HeLa Human Cell Line.

p38a phosphorylated at T180/Y182 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line, untreated or treated with calf intestinal phosphatase, using Rat Anti-Human Phospho-p38a (T180/Y182) Monoclonal Antibody (Catalog # MAB8692) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human phospho-p38 alpha (T180/Y182) Antibody (948243)

Application

Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line treated with calf intestinal phosphatase

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: p38 alpha

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses, such as UV irradiation and heat shock. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, Cdc42). p38 MAPK is involved in the regulation of Hsp27 and MAPKAP-2 and several transcription factors including ATF2, STAT1, and indirectly CREB via activation of MSK1. The p38 MAPK protein also plays a role in cell differentiation, autophagy and apoptosis. Mkk3 and SEK can activate p38 MAPK by phosphorylation at Thr180 and Tyr182, which in turn activates the MAPKAP kinase 2 and regulating phosphorylation of ATF2, Mac and MEF2.