Human phospho-Paxillin (Y31) Antibody (698239)

R&D Systems | Catalog # MAB61641

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 698239
View all Paxillin Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human Paxillin Y31 site

Specificity

Detects Phospho-Paxillin (Y31) in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human phospho-Paxillin (Y31) Antibody (698239)

Detection of Human Phospho-Paxillin (Y31) antibody by Western Blot.

Detection of Human Phospho-Paxillin (Y31) by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-Paxillin (Y31) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB61641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Phospho-Paxillin (Y31) at approximately 65 to 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Paxillin antibody in HUVEC Human Cells by Immunocytochemistry (ICC).

Paxillin in HUVEC Human Cells.

Paxillin phosphorylated at Y31 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Phospho-Paxillin (Y31) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB61641) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and focal adhesions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human phospho-Paxillin (Y31) Antibody (698239)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cell line

Western Blot

0.1 µg/mL
Sample: A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and Daudi human Burkitt's lymphoma cell line treated with Pervanadate (PV)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Paxillin

Paxillin is a 65 kDa cytoskeletal adaptor protein and member of the Paxillin family. Human Paxillin is 591 amino acids (aa) in length and contains four LIM zinc-binding domains. Alternative splicing produces three isoforms. Human Paxillin shares 94% and 85% aa identity with mouse and rat Paxillin, respectively. Paxillin is found at the interface between actin filaments and the plasma membrane, and it localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation at tyrosines 31 and 118 is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation.

Alternate Names

PXN

Entrez Gene IDs

5829 (Human); 19303 (Mouse); 360820 (Rat)

Gene Symbol

PXN

Additional Paxillin Products

Product Documents for Human phospho-Paxillin (Y31) Antibody (698239)

Certificate of Analysis

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Product Specific Notices for Human phospho-Paxillin (Y31) Antibody (698239)

For research use only

Citations for Human phospho-Paxillin (Y31) Antibody (698239)

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Protocols

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