Human Presenilin-1 N-Terminal Fragment Antibody (121121)

R&D Systems | Catalog # MAB149

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 121121
View all Presenilin-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Presenilin‑1 N-Terminal Fragment
Lys1-Lys80
Accession # P49768

Specificity

Detects human Presenilin‑1 N-Terminal Fragment in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Presenilin‑2 N-Terminal Fragment, rhPresenilin‑1 C-Terminal Fragment, or rhPresenilin‑2 C-Terminal Fragment.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Presenilin-1 N-Terminal Fragment Antibody (121121)

Presenilin-1 antibody in Human Brain by Immunohistochemistry (IHC-P).

Presenilin‑1 in Human Brain.

Presenilin-1 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Presenilin-1 N-Terminal Fragment Monoclonal Antibody (Catalog # MAB149) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Presenilin-1 N-Terminal Fragment Antibody (121121)

Application
Recommended Usage

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain and immersion fixed paraffin-embedded sections of human brain (medulla)

Western Blot

1 µg/mL
Sample: Recombinant Human Presenilin‑1 N-Terminal Fragment

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Presenilin-1

Presenilin‑1 (PS-1) is a multipass lysosomal membrane protein that is a component of the gamma secretase complex. It is cleaved by endoproteolysis to generate
N‑ and C‑terminal fragments. Some mutations in PS-1 are associated with the risk of early onset familial Alzheimer's disease.

Alternate Names

Presenilin1, PSEN1, S182

Entrez Gene IDs

5663 (Human)

Gene Symbol

PSEN1

UniProt

P49768

Additional Presenilin-1 Products

Product Documents for Human Presenilin-1 N-Terminal Fragment Antibody (121121)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Presenilin-1 N-Terminal Fragment Antibody (121121)

For research use only

Citations for Human Presenilin-1 N-Terminal Fragment Antibody (121121)

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Protocols

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