Human/Primate IL‑17/IL‑17A Biotinylated Antibody

R&D Systems | Catalog # BAF317

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human

Applications

Validated:

ELISA Detection (Matched Antibody Pair)

Cited:

Immunohistochemistry-Paraffin, ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Detection), Functional Assay, Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IL-17/IL-17A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552

Specificity

Detects human and primate IL-17 in direct ELISAs. In sandwich immunoassays, approximately 22% cross-reactivity with rhIL-17A/F heterodimer is observed, and less than 0.05% cross‑reactivity with recombinant mouse (rm) IL‑17, recombinant human (rh) IL-10, rhIL-12, rhIL-16, rhIFN-gamma, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E and rhIL-17F is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Primate IL‑17/IL‑17A Biotinylated Antibody

Human/Primate IL-17 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Primate IL‑17/IL‑17A Antibody (Catalog # MAB317)
  • Standard: Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF317 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17/IL-17A

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide-linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation. The non-glycosylated, E. coli-expressed rhIL-17 has been shown to be active on human as well as mouse cell lines.

References

  1. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  2. Yao, Z. et al. (1995) Immunity 3:811.
  3. Rouvier, E. et al. (1993) J. Immunol. 150:5445.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Gene Symbol

IL17A

UniProt

Q16552

Additional IL-17/IL-17A Products

Product Documents for Human/Primate IL‑17/IL‑17A Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Primate IL‑17/IL‑17A Biotinylated Antibody

For research use only

Citations for Human/Primate IL‑17/IL‑17A Biotinylated Antibody

Customer Reviews for Human/Primate IL‑17/IL‑17A Biotinylated Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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2 Stars
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1 Stars
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  • Human/Primate IL-17/IL-17A Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell culture supernatant
    Species: Human
    Verified Customer | Posted 08/22/2018
    The antibody was used as a secondary in an IL17A immunoassay.
    Human/Primate IL‑17/IL‑17A Biotinylated Antibody BAF317
  • Human/Primate IL-17/IL-17A Biotinylated Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 01/18/2018

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