Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Canine

Applications

Validated:

Immunohistochemistry, Neutralization, Intracellular Staining by Flow Cytometry, Dual RNAscope ISH-IHC Compatible, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Cell Culture, ELISA Detection, ELISA Development, Mass Cytometry, Imaging Mass Cytometry, Tissue Culture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-17/IL-17A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552

Specificity

Detects human IL-17 in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant canine IL-17 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL‑17/IL‑17A Antibody

IL-17/IL-17A antibody in Human Tonsil by Immunohistochemistry (IHC-P).

IL‑17/IL‑17A in Human Tonsil.

IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-17/IL-17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of IL‑17/IL‑17A in Human Crohn's Intestine

IL-17A mRNA (red) and IL17A protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human Crohn’s Disease Intestine. ACD’s Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-IL-17A (Catalog # 310931) and goat anti-human IL-17/IL-17A polyclonal antibody (R&D Systems Catalog # AF-317-NA) at 1ug/mL. Tissue was stained using RNAscope® 2.5 HD Detection Kit-RED (Catalog # 322360) and RNAscope® 2.5 LS Green Accessory Pack (Catalog # 322550). Tissue was counterstained with 50% hematoxylin (blue).
Detection of IL-17 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of IL‑17 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca 2+ionomycin for 16 hours, then stained with Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA, dark orange filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

IL-6 Secretion Induced by IL-17 and Neutralization by Human IL-17 Antibody.

IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody.

Recombinant Human IL-17 (317-ILB) stimulates IL-6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Human IL-17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). The ND50 is typically 0.02‑0.12 µg/mL.

Detection of Human IL-17/IL-17A by Immunohistochemistry

Detection of Human IL-17/IL-17A by Immunohistochemistry

Expression of psoriasis‐related cytokines in paradoxical psoriasiform reactions. Immunohistochemistry analysis performed on paradoxical skin lesions obtained from patients 1 (Pt1) and 2 (Pt2) shows similar values of IL‐17A+ cells, a reduction of dermal IFN‐ gamma + cells and an increase of IL‐22+ or IL‐36 gamma + cells, when compared with psoriatic skin lesions. LS and NLS skin of the same psoriatic patient (n = 3) was analyzed. Graphs show the mean of number of positive cells + SD per three sections. One out of three representative stainings is shown. *p < 0.01, **p < 0.05, versus classical psoriasis. Scale bars, 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31577850), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-17/IL-17A by Immunohistochemistry

Detection of Human IL-17/IL-17A by Immunohistochemistry

Inflammatory cell infiltrates of early acne lesions in immunohistochemistry.Representative immunohistochemical staining showing IL-17A+ cells in early acne lesion (a). Staining of IL-17A+ and CD3+ cells shows that IL-17A (in green) and CD3 (in red) were detected in acne vulgaris lesions. Cell nuclei were counterstained in blue. Arrows indicate IL-17A and CD3 double positive cells (b). T-bet+ cells (c) were more numerous than IL-17A+ cells or Foxp3+ cells (d) in the lesional acne skin. Large number of CD4+ cells (e), mostly lymphocytes, was seen around pilosebaceous unit and perivascularly. CD8+ cells (f) were fewer in number than CD4+ cells. Large number of CD68+ macrophages (g) and a few CD83+ cells (h), which are mature dendritic cells, were detected around sebaceous follicles. (Bar = 200 µm; in the insets bar  = 50 µm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25153527), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-17/IL-17A by Western Blot

Detection of IL-17/IL-17A by Western Blot

IL-17 upregulates FAP expression by activating the p-STAT3 signaling pathway.A Protein levels of FAP, pSTAT3 (Tyr705), pSTAT3 (Ser727), and STAT3 after 20 ng/ml IL-17a stimulation or PBS treatment. B Protein levels of FAP, IL-17, ACTA2, and VIMENTIN in IL-17-OE cells, FAP-OE cells, and the corresponding negative control cell lines. C Protein levels of pSTAT3 (Tyr705), pSTAT3 (Ser727), STAT3, pP65, and P65 in IL-17-OE cells, FAP-OE cells, and the corresponding negative control cell lines. D The binding ability of pSTAT3 (Tyr705) and STAT3 to the FAP promoter region in FAP-OE and FAP-NC cells was evaluated by a CUT&RUN assay. E The binding ability of pSTAT3 (Tyr705) and STAT3 to the FAP promoter region in IL-17-OE and IL-17-NC cells was evaluated by a CUT&RUN assay. F The mRNA expression of FAP of LX2 cell line after 24 h treatment of 50 μg/mL Colivelin. G The expression of FAP, STAT3, and p-STAT3 in LX2 cell line after 24 h treatment of 50 μg/mL Colivelin. H The FAP, STAT3, p-STAT3 level in FAP-OE and FAP-NC cell lines after 2 μM HJC0152 treatment. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41420-024-01995-4), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL‑17/IL‑17A Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Dual RNAscope ISH-IHC Compatible

0.5 - 5 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Crohn's intestine

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells treated with PHA

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells treated with PMA and Ca2+ ionomycin, fixed with paraformaldehyde, and permeabilized with saponin

Neutralization

Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.02-0.12 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.

Reviewed Applications

Read 9 reviews rated 4.2 using AF-317-NA in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-17/IL-17A

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Gene Symbol

IL17A

UniProt

Q16552

Additional IL-17/IL-17A Products

Product Documents for Human IL‑17/IL‑17A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IL‑17/IL‑17A Antibody

For research use only

Citations for Human IL‑17/IL‑17A Antibody

Customer Reviews for Human IL‑17/IL‑17A Antibody (9)

4.2 out of 5
9 Customer Ratings
5 Stars
22%
4 Stars
78%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human IL‑17/IL‑17A Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 5 的 9 reviews Showing All
Filter By:
Clear Filters X
  • Human IL-17/IL-17A Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Tonsil tissue
    Species: Human
    Verified Customer | Posted 09/13/2023
    FFPE heating antigen retrieval in pH9 primary Ab x100 in 1% BSA (IgG free) for 1 hour biotinylated rabbit anti-goat and streptavidin-HRP + DAB
    Human IL‑17/IL‑17A Antibody AF-317-NA
  • Human IL-17/IL-17A Antibody
    Name: Stephanie Zandee
    Application: Immunohistochemistry
    Sample Tested: Adult brain
    Species: Human
    Verified Customer | Posted 04/13/2018
    Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287 Used at 10ug/ml. Briefly, frozen brain sections were fixed in 4% PFA (Fisher Scientific), followed by antigen retrieval using heating in acid citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector). Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor Blocking Solution was added (Human TruStain FcX Biolegend, London, UK). Primary antibody was added overnight at 4 °C and detected with donkey anti-goat-biotin (ab6578, Abcam), followed by streptavidin-alkaline phosphatase (SA-5100, Vector) and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector).
    Human IL‑17/IL‑17A Antibody AF-317-NA
  • Human IL-17/IL-17A Antibody
    Name: BAREUN KIM
    Application: Block/Neutralize
    Sample Tested: 4T1 mouse breast cancer cell line and Breast tissue
    Species: mouse tumor-infiltrate lympocytes and Mouse
    Verified Customer | Posted 03/23/2018
  • Name: Nana Feinberg
    Verified Customer | Posted 04/21/2017
    Antigen retrieval pH 6.0 (20 min) Dilution-1:100, 1 h incubation. Detection with ImmPRESS HRP Anti-Goat Ig (Peroxidase) Polymer (# MP-7405-15 ) and TSA-Cy3 (#SAT704A001EA)
    Human IL‑17/IL‑17A Antibody AF-317-NA
  • Human IL-17/IL-17A Antibody
    Name: Nana Feinberg
    Application: Immunohistochemistry
    Sample Tested: Spleen tissue and Colon tissue
    Species: Human
    Verified Customer | Posted 04/21/2017
    Staining is done in Leica Bond autostainer. Epitope retrieval at pH 6 (20 min), IL17/IL17A dilution 1:100, incubation time-1h. Detected with TSA-Cy3
    Human IL‑17/IL‑17A Antibody AF-317-NA
  • Human IL-17/IL-17A Antibody
    Name: Manuel Flores
    Application: Immunocytochemistry
    Sample Tested: FFPE and FFPE human liver
    Species: Human
    Verified Customer | Posted 03/24/2017
    Multiplex immnunofluorescence labelling IL-17A, CD4, and FoxP3 on FFPE section of human liver biopsy. IL-17A primary Ab is the AF-317-NA from Novus Biologicals. Secondary Ab is A568. FFPE sections were dewaxed with xylene and rehydrated with ethanol solutions (decreasing %). Antigen retrieval was performed with sodium citrate pH6 in a pressure cooker. IL-17A signal is specific, and the staining pattern is what you would expect for a molecule that is produced in the cytoplasm and secreted.
    Human IL‑17/IL‑17A Antibody AF-317-NA
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 20426778
    Species: Human
    Verified Customer | Posted 01/07/2015
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 20357258
    Species: Other
    Verified Customer | Posted 01/07/2015
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: See PMID 21307293
    Species: Human
    Verified Customer | Posted 01/07/2015

There are no reviews that match your criteria.

Showing  1 - 5 的 9 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies