IL-17/IL-17A Antibody (41809)

R&D Systems | Catalog # MAB317

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, ELISA Capture (Matched Antibody Pair), Neutralization, Dual RNAscope ISH-IHC Compatible, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Flow Cytometry, ELISA Capture, ELISA Development, ELISA Development (Capture), Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 41809
View all IL-17/IL-17A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552

Specificity

Detects human and primate IL-17 in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17 is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for IL-17/IL-17A Antibody (41809)

IL-17/IL-17A antibody in Human Crohn's Disease Intestine by Immunohistochemistry (IHC-P).

IL‑17/IL‑17A in Human Crohn's Disease Intestine.

IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human/Primate IL-17/IL-17A Monoclonal Antibody (Catalog # MAB317) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Immunoprecipitation of Human IL‑17.

Immunoprecipitation of Human IL‑17.

Human IL-17 was immunoprecipitated from 100 µg of human primary differentiated Th17 cell lysate following incubation with 3 µg Mouse Anti-Human/Primate IL-17 Monoclonal Antibody (Catalog # MAB317) or isotype control antibody (Catalog # MAB004) overnight at 4 °C. IL-17-antibody complexes were absorbed using anti-mouse agarose beads. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.
IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody.

IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody.

Recombinant Human IL-17 (Catalog # 317-ILB) stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Human IL-17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-17 Monoclonal Antibody (Catalog # MAB317). The ND50 is typically 1-3 µg/mL.

Detection of IL‑17/IL‑17A in Human Crohn's Intestine.

Formalin-fixed paraffin-embedded tissue sections of human Crohn’s Disease Intestine were probed for IL-17A mRNA (ACD RNAScope Probe, catalog #310931; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human IL-17A monoclonal antibody (R&D Systems catalog # MAB317) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.
Human IL-17 / IL-17A ELISA Standard Curve

Human IL-17 / IL-17A ELISA Standard Curve

Recombinant Human IL‑17/IL‑17A (Catalog # 317-ILB) was serially diluted and captured by Mouse Anti-Human/Primate IL‑17/IL‑17A Monoclonal Antibody (Catalog # MAB317) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑17/IL‑17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for IL-17/IL-17A Antibody (41809)

Application
Recommended Usage

Dual RNAscope ISH-IHC Compatible

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human crohn's intestine

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Crohn's disease intestine

Immunoprecipitation

3 µg/100 µg cell lysate
Sample: Human primary differentiated Th17 cells

Neutralization

Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.

Human/Primate IL-17 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human/Primate IL‑17/IL‑17A Biotinylated Antibody (Catalog # BAF317)
  • Standard: Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using MAB317 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17/IL-17A

IL-17 is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Gene Symbol

IL17A

UniProt

Q16552

Additional IL-17/IL-17A Products

Product Documents for IL-17/IL-17A Antibody (41809)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for IL-17/IL-17A Antibody (41809)

For research use only

Citations for IL-17/IL-17A Antibody (41809)

Customer Reviews for IL-17/IL-17A Antibody (41809) (3)

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Customer Images

Showing  1 - 3 的 3 reviews Showing All
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  • Human/Primate IL-17/IL-17A Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Ischemic brain tissue
    Species: Human
    Verified Customer | Posted 08/19/2021
    IL-17/IL-17A Antibody (41809) MAB317
  • Human/Primate IL-17/IL-17A Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell culture supernatant
    Species: Human
    Verified Customer | Posted 08/22/2018
    Use the antibody to coat the wells in an immunoassay.
    IL-17/IL-17A Antibody (41809) MAB317
  • Human/Primate IL-17/IL-17A Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 01/18/2018

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Protocols

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