Human Rab13 Antibody

Detection of Human Rab13 by Western Blot. Western blot shows lysates of human lung tissue and human aorta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Rab13 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Rab13 in Caco‑2 Human Cell Line. Rab13 was detected in immersion fixed Caco-2 human colorectal adenocarcinoma cell line using Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Shows Rab13 Specificity Using Knockout Cell Line. Western blot shows lysates of HEK-293T parental cell line and Rab13 knockout HEK-293T cell line (KO). Nitrocellulose membrane was probed with Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated secondary antibody. A specific band was detected for Rab13 at approximately 23 kDa (as indicated) in the parental HEK-293T cell line, but is not detectable in knockout HEK-293T cell line. Primary antibody dilution used: 2.5 µg/mL. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Western Blot Shows Rab13 Specificity Using Knockdown Cell Line. Western blot shows lysates of U87 MG parental cell line and Rab13 knockout U87 MG cell line (KD). Nitrocellulose membrane was probed with Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated secondary antibody. A specific band was detected for Rab13 at approximately 23 kDa (as indicated) in the parental U87 MG cell line, but is significantly reduced in the U87 MG cell line. Primary antibody dilution used: 2.5 µg/mL. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Rab13 Specificity is Shown by Immunocytochemistry in Knockdown Cell Line. U‑87 MG human glioblastoma/astrocytoma parental cell line WT and Rab13 U-87 MG KD cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KD cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KD cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1 µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).