PGD2 Synthase/PTGDS Antibody (997303)

R&D Systems | Catalog # MAB10099

R&D Systems

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 997303
View all PGD2 Synthase/PTGDS Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PGD2 Synthase/PTGDS
Met1-Gln190
Accession # P41222

Specificity

Detects human and rat PGD2 Synthase/PTGDS in direct ELISA and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for PGD2 Synthase/PTGDS Antibody (997303)

Detection of Human and Rat PGD2 Synthase/PTGDS antibody by Western Blot.

Detection of Human and Rat PGD2 Synthase/PTGDS by Western Blot.

Western blot shows lysates of human heart tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Rat PGD2 Synthase/PTGDS Monoclonal Antibody (Catalog # MAB10099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PGD2 Synthase/PTGDS at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PGD2 Synthase/PTGDS antibody in Human Brain by Immunohistochemistry (IHC-P).

PGD2 Synthase/PTGDS in Human Brain.

PGD2 Synthase/PTGDS was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human/Rat PGD2 Synthase/PTGDS Monoclonal Antibody (Catalog # MAB10099) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for PGD2 Synthase/PTGDS Antibody (997303)

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Western Blot

0.5 µg/mL
Sample: Human heart tissue and Rat brain tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PGD2 Synthase/PTGDS

Prostaglandin-H2 D-isomerase or PGD2 synthase (PTGDS) is an enzyme that catalyzes the conversion of Prostaglandin H2 (PGH2) to Prostaglandin D2 (PGD2), a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Prostaglandin D2 synthase is used clinically as a diagnostic marker for Cerebrospinal fluid rhinorrhea, important in the assessment of head trauma severity. It has been also proposed that the urinary and serum levels pf PTGDS may be also a marker of renal damage in diabetes mellitus and hypertension.

Long Name

Prostaglandin D2 Synthase

Alternate Names

Cerebrin-28, PDS, PGD2, PGDS2, PH2DISO, PTGDS

Entrez Gene IDs

5730 (Human); 25526 (Rat)

Gene Symbol

PTGDS

UniProt

P41222

Additional PGD2 Synthase/PTGDS Products

Product Documents for PGD2 Synthase/PTGDS Antibody (997303)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for PGD2 Synthase/PTGDS Antibody (997303)

For research use only

Citations for PGD2 Synthase/PTGDS Antibody (997303)

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Protocols

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