Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all S100A8 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100A8
Met1-Glu93
Accession # P05109

Specificity

Detects human S100A8 in direct ELISAs and Western blots. In these formats, less than 1% cross-reactivity with recombinant mouse S100A8, recombinant human (rh) S100A1, and rhS100P is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human S100A8 Antibody

Detection of Human S100A8 antibody by Western Blot.

Detection of Human S100A8 by Western Blot.

Western blot shows lysates of human brain tissue, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4570) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

S100A8 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

S100A8 in HeLa Human Cell Line.

S100A8 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4570) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of S100A8 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of S100A8 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4570, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of Human S100A8 antibody by Simple WesternTM.

Detection of Human S100A8 by Simple WesternTM.

Simple Western lane view shows lysates of normal adjacent tissue and Crohns tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A8 at approximately 10 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4570) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human S100A8 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line, fixed with paraformaldehyde and permeabilized with saponin

Simple Western

10 µg/mL
Sample: Normal adjacent tissue and Crohns tissue

Western Blot

1 µg/mL
Sample: Human brain tissue, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 2 reviews rated 4 using AF4570 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A8

S100A8 (also known as MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca‑dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12-47 and 46-81) and one high-affinity Ca‑binding site (aa 59-70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP‑2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Long Name

S100 Calcium Binding Protein A8

Alternate Names

Calgranulin A, CFAG, MRP8

Entrez Gene IDs

6279 (Human); 20201 (Mouse)

Gene Symbol

S100A8

UniProt

P05109

Additional S100A8 Products

Product Documents for Human S100A8 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S100A8 Antibody

For research use only

Citations for Human S100A8 Antibody

Customer Reviews for Human S100A8 Antibody (2)

4 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
50%
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1 Stars
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Customer Images

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  • Human S100A8 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 07/01/2016
    S100A8 Antibody tested against different concentrations of human recombinant S100A8/A9.
    Human S100A8 Antibody AF4570
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 20806971
    Species: Human
    Verified Customer | Posted 01/08/2015

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