Human Serpin B8/Proteinase Inhibitor 8 Antibody (423023)

R&D Systems | Catalog # MAB4158

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 423023
View all Serpin B8/Proteinase Inhibitor 8 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf21-derived recombinant human Serpin B8/Proteinase Inhibitor 8
Asp2-Pro374
Accession # P50452

Specificity

Detects human Serpin B8/Proteinase Inhibitor 8 in direct ELISAs. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) Serpin B8 is observed. No cross-reactivity with recombinant human Serpin A1, A3, A4, A5, A6, A9, A11, A12, B3, B6, B9, C1, D1, E1, E2, F1, F2, G1, I1, I2, rmSerpin A1, A3N, A6, A9, C1, D1, E1, E2, F1, F2, I1, recombinant rat (rr) Serpin A6, or rrSerpin A12 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Serpin B8/Proteinase Inhibitor 8 Antibody (423023)

Serpin B8/Proteinase Inhibitor 8 antibody in Human Pancreas by Immunohistochemistry (IHC-P).

Serpin B8/Proteinase Inhibitor 8 in Human Pancreas.

Serpin B8/Proteinase Inhibitor 8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Human Serpin B8 Monoclonal Antibody (Catalog # MAB4158) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Serpin B8/Proteinase Inhibitor 8 Antibody (423023)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Reviewed Applications

Read 1 review rated 5 using MAB4158 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin B8/Proteinase Inhibitor 8

Serpin B8 (serine proteinase inhibitor-clade B #8; also PI8) is a 42‑45 kDa, cytoplasmic and secreted member of the ovalbumin (clade B)-subfamily, Serpin superfamily of protease inhibitors. It is produced by multiple cell types and shows inhibitory activity towards furin and other proteinases such as chymotrypsin. Human Serpin B8 is 374 amino acids (aa) in length. Although it has no signal sequence, it is released by platelets. The active inhibitor site lies between Arg336 and Arg342. One potential isoform shows a 2 aa substitution for the C-terminal 134 amino acids. Full length human Serpin B8 shares 78% and 83% aa identity with mouse and canine Serpin B8, respectively.

Alternate Names

CAP2, NK10, PI8

Entrez Gene IDs

5271 (Human); 20725 (Mouse); 288937 (Rat)

Gene Symbol

SERPINB8

UniProt

P50452

Additional Serpin B8/Proteinase Inhibitor 8 Products

Product Documents for Human Serpin B8/Proteinase Inhibitor 8 Antibody (423023)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serpin B8/Proteinase Inhibitor 8 Antibody (423023)

For research use only

Related Research Areas

Serine Proteases and Regulators

Customer Reviews for Human Serpin B8/Proteinase Inhibitor 8 Antibody (423023) (1)

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  • Human Serpin B8/Proteinase Inhibitor 8 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Pancreas tissue
    Species: Human
    Verified Customer | Posted 12/16/2021
    Human Serpin B8/Proteinase Inhibitor 8 Antibody (423023) MAB4158

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Protocols

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