Human Sirtuin 1/SIRT1 Antibody

Catalog # Availability Size / Price Qty
Sirtuin 1/SIRT1 in HepG2 Human Cell Line.
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Product Details
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Human Sirtuin 1/SIRT1 Antibody Summary

Species Reactivity
Detects human Sirtuin 1/SIRT1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Sirtuin 2, 3, 5, or 6 is observed.
Monoclonal Mouse IgG1 Clone # 834918
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Sirtuin 1/SIRT1
Accession # Q96EB6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Sirtuin 1/SIRT1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

Sirtuin 1/SIRT1 in HepG2 Human Cell Line. Sirtuin 1/SIRT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Sirtuin 1/SIRT1 Monoclonal Antibody (Catalog # MAB7714) at 25 µg/mL for 1 hour at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of Sirtuin 1/SIRT1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Sirtuin 1/SIRT1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Sirtuin 1/SIRT1 Monoclonal Antibody (Catalog # MAB7714, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sirtuin 1/SIRT1

SIRT1 (SIR2-like protein 1; also NAD-dependent protein deacetylase sirtuin-1 and hSIR2) is a class I member of the sirtuin family of enzymes. Although its predicted MW is 81 kDa, it runs anomalously at 110-120 kDa in SDS-PAGE. It is a widely expressed nuclear protein that participates in the deacetylation of multiple proteins, including p300, p53, LKB1 and histone H1. Functionally, this has the effect of promoting heterochromatin formation, cell survival and resistance to oxidative stress. Metabolically, SIRT1 induces insulin secretion, inhibits glycolysis and suppresses fatty acid synthesis. Human SIRT1 is 747 amino acids (aa) in length. It possesses two NLSs (aa 32-39 and 223-230), an NES (aa 138-145), and a sirtuin-type deacetylase domain (aa 241-495) that contains an NAD and Zn binding motif. There are at least 12 utilized Ser/Thr phosphorylation sites, plus two nitrosylated Cys and one acetylated Ala. There are also four potential isoform variants. One is 95 kDa in size and shows a deletion of aa 454-639, a second is 17 kDa in size and contains a 16 aa substitution for aa 149-747, and a third contains an alternative start site at Met296. SIRT1 is also known to undergo proteolysis by cathepsin B at Val533Ser534, generating a fourth, C-terminally truncated 75 kDa isoform. Full-length SIRT1 is suggested to form trimers, while the 17 kDa isoform appears to form dimers. Over aa 2-747, human and mouse SIRT1 share 86% aa sequence identity.

Long Name
Silent Mating Type Information Regulation 2 Homolog
Entrez Gene IDs
23411 (Human); 93759 (Mouse); 309757 (Rat)
Alternate Names
EC 3.5.1; S. cerevisiae, homolog) 1; SIR2alpha; SIR2L1; SIR2-like protein 1; SIRT1; Sirtuin 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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