Human STEAP2 Antibody Summary
Accession # Q8NFT2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of STEAP2 in Human Prostate. STEAP2 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human STEAP2 Monoclonal Antibody (Catalog # MAB11742) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of STEAP2 in Human Prostate Cander. STEAP2 was detected in immersion fixed paraffin-embedded sections of human prostate cander using Mouse Anti-Human STEAP2 Monoclonal Antibody (Catalog # MAB11742) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of STEAP2 in LNCaP cells. STEAP2 was detected in immersion fixed LNCaP human prostate cancer cell line (Positive) and absent in MCF‑7 human breast cancer cell line (Negative) using Mouse Anti-Human STEAP2 Monoclonal Antibody (Catalog # MAB11742) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STEAP2
Six-transmembrane epithelial antigen of the prostate 2 (STEAP2) is a member of the STEAP family of metalloreductases, with a molecular weight of approximately 49 kDa. STEAP2 is an integral membrane protein that plays a role in cellular homeostasis by facilitating the reduction of metal ions such as iron and copper, which are critical cofactors for various enzymatic processes. STEAP2 is highly expressed in prostate tissue and is also detected at lower levels in other tissues, supporting its emerging role in tissue-specific metabolic regulation. Overexpression of STEAP2 has been implicated in prostate cancer progression, where it contributes to the proliferation, migration, and invasiveness of tumor cells. The dysregulation of STEAP2 expression has also been associated with other malignancies, suggesting its broader significance in cancer biology. Additionally, STEAP2 has garnered interest as a potential biomarker for cancer diagnosis and prognosis, as well as a therapeutic target, particularly in prostate and other hormone-regulated cancers.
- Hubert RS, Vivanco I, Chen E, Rastegar S, Leong K, Mitchell SC, Madraswala R, Zhou Y, Kuo J, Raitano AB, Jakobovits A, Saffran DC, Afar DE. STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors. Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14523-8. doi: 10.1073/pnas.96.25.14523. PMID: 10588738; PMCID: PMC24469.
- Ohgami RS, Campagna DR, McDonald A, Fleming MD. The Steap proteins are metalloreductases. Blood. 2006 Aug 15;108(4):1388-94. doi: 10.1182/blood-2006-02-003681. Epub 2006 Apr 11. PMID: 16609065; PMCID: PMC1785011.
- Gomes IM, Maia CJ, Santos CR. STEAP proteins: from structure to applications in cancer therapy. Mol Cancer Res. 2012 May;10(5):573-87. doi: 10.1158/1541-7786.MCR-11-0281. Epub 2012 Apr 20. PMID: 22522456.
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