Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Canine

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-reported

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Labeling

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # TRA-1-60
View all TRA-1-60(R) Primary Antibodies »

Product Specifications

Immunogen

2102Ep human embryonal carcinoma cell line

Specificity

Detects human TRA‑1‑60(R).

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human TRA‑1‑60(R) Neuraminidase Resistant Epitope Antibody

Detection of TRA-1-60 antibody in BG01V Human Cells antibody by Flow Cytometry

Detection of TRA‑1‑60 in BG01V Human Cells by Flow Cytometry

BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770, filled histogram) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (F0116).
TRA-1-60 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

TRA‑1‑60 in BG01V Human Stem Cells.

TRA-1-60 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (yellow; NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TRA-1-60(R) antibody in ADLF1 and FAB2 Stem Cell Lines by Immunocytochemistry (ICC).

TRA‑1‑60(R) in ADLF1 and FAB2 Stem Cell Lines.

TRA-1-60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Mouse Anti-Human TRA-1-60(R) Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Detection of TRA‑1‑60(R) in iPSC cells by Flow Cytometry.

iPSC cells were stained with Mouse Anti-Human TRA‑1‑60(R) Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770, filled histogram) or isotype control antibody Mouse IgM (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.
Detection of Canine TRA-1-60(R) by Flow Cytometry

Detection of Canine TRA-1-60(R) by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine TRA-1-60(R) by Flow Cytometry

Detection of Canine TRA-1-60(R) by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody by Flow Cytometry

Detection of Canine Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody by Flow Cytometry

Detection of Canine Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine TRA-1-60(R) by Flow Cytometry

Detection of Canine TRA-1-60(R) by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine TRA-1-60(R) by Flow Cytometry

Detection of Canine TRA-1-60(R) by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine TRA-1-60(R) by Flow Cytometry

Detection of Canine TRA-1-60(R) by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine TRA-1-60(R) by Flow Cytometry

Detection of Canine TRA-1-60(R) by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine TRA-1-60(R) Neuraminidase Resistant Epitope by Flow Cytometry

Detection of Canine TRA-1-60(R) Neuraminidase Resistant Epitope by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine TRA-1-60(R) Neuraminidase Resistant Epitope by Flow Cytometry

Detection of Canine TRA-1-60(R) Neuraminidase Resistant Epitope by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TRA‑1‑60(R) Neuraminidase Resistant Epitope Antibody

Application
Recommended Usage

CyTOF-reported

This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: BG01V human embryonic stem cells and iPSC cells

Immunocytochemistry

8-25 µg/mL
Sample: Immersion-fixed BG01V human embryonic stem cells immersion fixed ADLF1 and FAB2 induced pluripotent stem cell lines

Reviewed Applications

Read 1 review rated 5 using MAB4770 in the following applications:

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Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRA-1-60(R)

TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2‑4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).

References

  1. Andrews, P. et al. (1984) Hybridoma 3:347.
  2. Thomson, J. et al. (1998) Science 282:1145.
  3. Giwercman, A. et al. (1993) Cancer 72:1308.
  4. Marrink, J. et al. (1991) Int. J. Cancer 49:368.
  5. Badcock, G. et al. (1999) Cancer Res. 59:4715.
  6. Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.

Long Name

Tumor-related Antigen-1-60 [R]

Alternate Names

TRA160(R)

Entrez Gene IDs

5420 (Human)

Gene Symbol

PODXL

Additional TRA-1-60(R) Products

Product Documents for Human TRA‑1‑60(R) Neuraminidase Resistant Epitope Antibody

Certificate of Analysis

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Product Specific Notices for Human TRA‑1‑60(R) Neuraminidase Resistant Epitope Antibody

For research use only

Citations for Human TRA‑1‑60(R) Neuraminidase Resistant Epitope Antibody

Customer Reviews for Human TRA‑1‑60(R) Neuraminidase Resistant Epitope Antibody (1)

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  • Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: H9 human embryonic stem cells
    Species: Human
    Verified Customer | Posted 10/20/2017
    The cells were stained with 1 µg of TRA-1-60 antibody in 100 µl FACS buffer at 4C for 30 min. After washing, the cells were incubated with anti-IgM-AF488 antibody at 4C for 20 min.
    Human TRA‑1‑60(R) Neuraminidase Resistant Epitope Antibody MAB4770

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