Human VAP-A Antibody (604101)

R&D Systems | Catalog # MAB5820

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 604101
View all VAP-A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VAP-A
Ala2-Met132
Accession # Q9P0L0

Specificity

Detects human VAP‑A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human VAP-B or recombinant rat VAP-B is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human VAP-A Antibody (604101)

Detection of Human VAP-A antibody by Western Blot.

Detection of Human VAP-A by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human VAP-A Monoclonal Antibody (Catalog # MAB5820) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAP-A at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1with 0.05% Tween 20.
VAP-A antibody in Human Brain by Immunohistochemistry (IHC-P).

VAP‑A in Human Brain.

VAP-A was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human VAP-A Monoclonal Antibody (Catalog # MAB5820) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human VAP-A Antibody (604101)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VAP-A

Vesicle-associated membrane protein (VAMP)-associated protein A (VAP-A; also VAMP-A and VAP-33) is a 33 kDa, ubiquitously expressed, type IV transmembrane protein belonging to the VAP family of proteins (1). It is found in plasma and ER membranes as well as in intracellular vesicles as a homodimer and a heterodimer with VAP-B. Human VAP-A is synthesized as a 249 amino acid (aa) precursor that contains a 227 aa cytoplasmic domain and a 21 aa transmembrane region. The cytoplasmic domain contains a mobile sperm protein (MSP) domain (aa 13-131) and a coiled-coil region (aa 169-205). Human VAP-A is 97% aa identical to mouse and rat VAP-A. VAP-A and VAP-B recruit FFAT (two phenylalanines in an acidic tract)-motif-containing proteins to the cytosolic surface of ER membranes through a conserved region within their MSP domain, and they have been implicated in regulation of membrane transport, phospholipid biosynthesis, and the unfolded protein response (2, 3). Their ability to interact with lipid-transfer/binding proteins (LT/BPs) may affect the lipid composition of certain cellular membranes (2, 4). VAPs play a critical role in maintaining the structural and functional properties of the Golgi complex (2). Knockdown of VAP reduces the levels of phosphatidylinositol‑4‑phosphate (PI4P), diacylglycerol (DAG), and sphingomyelin (SM) in Golgi membranes and exports pleiotropic effects in Golgi-mediated transport (2). The effects of VAPs are mediated by their interacting FFAT-motif-containing proteins Nir2, OSBP, and CERT (2). VAPs provide a scaffold for these LT/BPs at the ER-Golgi membrane contact sites, thereby affecting the lipid composition of the Golgi membranes and consequently their structural and functional identities (2). VAP-A associates with and regulates the neurite outgrowth-promoting activity of protrudin, a protein that promotes neurite formation (5).

References

  1. Weir, M.L. et al. (1998) Biochem. J. 333:247.
  2. Peretti, D. et al. (2008) Mol. Biol. Cell 19:3871.
  3. Kaiser, S.E. et al. (2005) Structure 13:1035.
  4. Loewen, C.J. et al. (2003) EMBO J. 22:2025.
  5. Saita, S. et al. (2009) J. Biol. Chem. 284:13766.

    Long Name

    VAMP [Vesicle-associated Membrane Protein]-associated Protein A

    Alternate Names

    VAMP-A, VAP-33, VAPA

    Entrez Gene IDs

    9218 (Human); 30960 (Mouse); 58857 (Rat)

    Gene Symbol

    VAPA

    UniProt

    Q9P0L0 (Human)

    Additional VAP-A Products

    Product Documents for Human VAP-A Antibody (604101)

    Certificate of Analysis

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    Product Specific Notices for Human VAP-A Antibody (604101)

    For research use only

    Citations for Human VAP-A Antibody (604101)

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