Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 639925
View all ZEB1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ZEB1
Glu430-Ser575
Accession # P37275

Specificity

Detects human ZEB1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ZEB1 Antibody

Detection of ZEB1 in Human Pancreas.

ZEB1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB11504) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of ZEB1 in Human Skeletal Muscle.

ZEB1 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB11504) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human ZEB1 Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas and human skeletal muscle

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZEB1

Zinc finger E-box-binding homeobox 1 (ZEB1; also transcription factor 8 (TCF-8)) is a 124 kDa member of the delta-EF1/ZFH-1 C2H2-type zinc finger family.  Human ZEB1 is 1124 amino acids (aa) in length. The protein contains seven C2H2-type zinc fingers and one homeobox DNA-binding domain. In addition, there are eight phosphoserines and one phosphothreonine. Residues 989-1124 make up a glutamine-rich area. Within aa 430-575, human ZEB1 shares 84% and 82% aa sequence identity with mouse and rat ZEB1, respectively. The protein is expressed in heart and skeletal muscle, and defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3, a rare disease involving metaplasia and overgrowth of the corneal endothelial cells.

Long Name

Zinc Finger E-box Binding Homeobox 1

Alternate Names

AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP

Entrez Gene IDs

6935 (Human); 21417 (Mouse); 25705 (Rat)

Gene Symbol

ZEB1

UniProt

P37275

Additional ZEB1 Products

Product Documents for Human ZEB1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human ZEB1 Antibody

For research use only

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Protocols

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