Mouse IgG1 Isotype Control (MG1)

Novus Biologicals | Catalog # NBP1-97005

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA, Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, SDS-Page

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MG1
View all IgG1 Isotype Controls »

Product Specifications

Specificity

Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG1 and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.

Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Mouse IgG1 Isotype Control (MG1)

SDS-PAGE: Mouse IgG1 Isotype Control (MG1) [NBP1-97005]

SDS-PAGE: Mouse IgG1 Isotype Control (MG1) [NBP1-97005]

SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.

Applications for Mouse IgG1 Isotype Control (MG1)

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:1000 - 1:5000

Fluorophore-linked immunosorbent assay

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This product has been tested by ELISA and SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Reviewed Applications

Read 1 review rated 5 using NBP1-97005 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG1

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

Additional IgG1 Products

Product Documents for Mouse IgG1 Isotype Control (MG1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Mouse IgG1 Isotype Control (MG1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Citations for Mouse IgG1 Isotype Control (MG1)

Customer Reviews for Mouse IgG1 Isotype Control (MG1) (1)

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Customer Images

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: human fetal derived neural stem cells
    Species: Human
    Verified Customer | Posted 04/21/2015
    MHC-II partial positivity
    Mouse IgG1 Isotype Control (MG1) NBP1-97005

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Protocols

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FAQs for Mouse IgG1 Isotype Control (MG1)

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    • Q: Could you please let me know the supply form (Liquid or Lyohpilized) of this antibody? 
      A: Yes, it is liquid. Supplied in 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: none. Preservative: 0.01% Sodium Azide. 

    • A: This isotype control was purified from ascites and consists of antibody from Balb/c with possible trace amounts from Swiss webster.
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