MMR/CD206/Mannose Receptor Antibody (JF0953)

Novus Biologicals | Catalog # NBP2-66956

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF0953 expressed in HEK293
View all MMR/CD206 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human MMR/CD206/Mannose Receptor aa 1,407-1,456 / 1,456. (SwissProt: P22897 Human; SwissProt: Q61830 Mouse)

Localization

Endosome membrane, Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMR/CD206/Mannose Receptor Antibody (JF0953)

Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Lane 1: Wild-type HepG2 whole cell lysate (10 ug).Lane 2: CD206 knockdown HepG2 whole cell lysate (10 ug).Lane 3: CD206 knockdown HepG2 whole cell lysate (10 ug).NBP2-66956 was shown to specifically react with CD206 in wild-type Hela cells. Weakened bands were observed when CD206 knockdown samples were tested. Wild-type and CD206 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Immunocytochemistry/ Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of MMR/CD206/Mannose Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse lung tissue lysate Lane 2: D3 cell lysate
Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Human lung tissue lysate Lane 2: HepG2 cell lysate
Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: 293T cell lysateLane 2: MCF-7 cell lysate
Immunocytochemistry/ Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Immunocytochemistry/ Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Immunocytochemistry/ Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
MMR/CD206/Mannose Receptor Antibody (JF0953) Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956]

Western blot analysis on different lysates with NBP2-66956 at 1/5,000 dilution. Lane 1: MOLT-4 cell lysate (20 µg/Lane) Lane 2: Mouse lung tissue lysate (40 µg/Lane) Predicted band size: 166 kDa Observed band size: 166 kDa Exposure time: 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66956 at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for MMR/CD206/Mannose Receptor Antibody (JF0953)

Application
Recommended Usage

Flow Cytometry

1:500-1:1000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:1000-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMR/CD206

The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail.The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines such as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The antibody 15-2 prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.

Long Name

Macrophage Mannose Receptor

Alternate Names

CD206, CLEC13D, MRC1

Gene Symbol

MRC1

Additional MMR/CD206 Products

Product Documents for MMR/CD206/Mannose Receptor Antibody (JF0953)

Certificate of Analysis

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Product Specific Notices for MMR/CD206/Mannose Receptor Antibody (JF0953)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MMR/CD206/Mannose Receptor Antibody (JF0953)

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Protocols

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