Mouse BMP-4 Antibody (1128D)

R&D Systems | Catalog # MAB5020

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1128D
View all BMP-4 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived mature mouse BMP-4
Ser293-Arg408
Accession # P21275

Specificity

Detects mouse BMP-4 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse BMP-4 Antibody (1128D)

Detection of Mouse BMP-4 antibody by Western Blot.

Detection of Mouse BMP‑4 by Western Blot.

Western blot shows Recombinant Mouse BMP-4 (Catalog # 5020-BP) and lysates of mouse embryo tissue. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB5020) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for BMP-4 at approximately 40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
BMP-4 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

BMP‑4 in Mouse Embryo.

BMP-4 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rabbit Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB5020) at 2 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastrointestinal tract cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of BMP-4 by Western Blot

Detection of BMP-4 by Western Blot

BMPR1a deficiency resulted in reduced myoepithelial lineages and compromised repopulating capacity of mammary stem cells. (A) FACS analysis of mammary gland epithelial cells from control and cKO mice at pregnancy day 14.5 using the Lin/CD24/CD29/CD61 surface marker set. n = 4 mice per group. (B) Quantification of Lin– CD24+CD29high (Myo), Lin– CD24+CD29low (Lu) and Lin– CD24+CD29lowCD61+ cell populations in control and cKO mice at pregnancy day 14.5. n = 4 biological replicates. (C) Immunofluorescence staining for K14 (red) and K8 (green) in control and cKO mammary glands at pregnancy day 14.5. n = 3 mice per group. Scale bar, 25 μm. (D) Wstern blotting for K14 in control and cKO mammary glands at pregnancy day 14.5. beta -Actin was used as a loading control. Statistical analysis the expression of K14/ beta -Actin. n = 3 mice. (E) Immunofluorescence staining for K14 (red) and K8 (green) in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. n = 3 biological replicates. Scale bar, 25 μm. (F) Wstern blotting for K14 in HC11 cells treated with BMP4 (50 ng/mL) for 24 h. beta -Actin was used as a loading control. Statistical analysis the expression of K14/ beta -Actin. n = 4 biological replicates. (G) Illustration of the mammary transplantation assays using K14-rtTA/teto-Cre/Bmpr1afl/fl and control mouse mammary stem cells and the induction strategy. (H) Whole-mount staining of mammary glands from the mammary transplantation assays and quantification of the proportion of ductal trees. n = 5 mice per group. Scale bar, 1 mm. (I) Immunohistochemistry staining for Integrin beta 3 in control and cKO mammary glands at pregnancy day 14.5. Quantification of Integrin beta 3+ cells in alveoli. n = 3 mice per group. Scale bar, 50 μm. Data were presented as means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34336839), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse BMP-4 Antibody (1128D)

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)

Western Blot

0.25 µg/mL
Sample: Mouse embryo tissue

Reviewed Applications

Read 2 reviews rated 4.5 using MAB5020 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-4

BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). The mouse BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). The propeptide is cleaved intracellularly by furin or proprotein convertase 6, enabling the 15 kDa mature BMP-4 monomer to form an active disulfide linked homodimer or heterodimer with BMP-7 (7-9). Mature mouse and human BMP-4 share 98% aa sequence identity. Mouse BMP-4 shares 85% aa sequence identity with mouse BMP-2 and 35%-54% with other mouse BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13-15).

References

  1. Zhang, P. et al. (2008) Blood 111:1933.
  2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
  5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
  6. Oida, S. et al. (1995) DNA Seq. 5:273.
  7. Cui, Y. et al. (1998) EMBO J. 17:4735.
  8. Cui, Y. et al. (2001) Genes Dev. 15:2797.
  9. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  11. Chen, D. et al. (2004) Growth Factors 22:233.
  12. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
  13. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
  15. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Entrez Gene IDs

652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)

Gene Symbol

BMP4

UniProt

P21275 (Mouse)

Additional BMP-4 Products

Product Documents for Mouse BMP-4 Antibody (1128D)

Certificate of Analysis

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Product Specific Notices for Mouse BMP-4 Antibody (1128D)

For research use only

Citations for Mouse BMP-4 Antibody (1128D)

Customer Reviews for Mouse BMP-4 Antibody (1128D) (2)

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  • Mouse BMP-4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Pituitary gland tissue
    Species: Mouse
    Verified Customer | Posted 10/30/2021
    Mouse BMP-4 Antibody (1128D) MAB5020
  • Mouse BMP-4 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: E11.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 12/02/2020
    Antibody was used to stain heart region on E11.5 mouse embryo.
    Mouse BMP-4 Antibody (1128D) MAB5020

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Protocols

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