Mouse Collagen I alpha 1 Antibody (1102924)

R&D Systems | Catalog # MAB11700

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western, COMET

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 1102924
View all Collagen I alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # P11087

Specificity

Detects a synthetic peptide specific for mouse COL1A1 around amino acid 215 in Direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Collagen I alpha 1 Antibody (1102924)

COL1A1 in Mouse Colon via seqIF™ staining on COMET™

COL1A1 was detected in immersion fixed paraffin-embedded sections of mouse Colon using Rat Anti-Mouse COL1A1, Monoclonal Antibody (Catalog #MAB11700) at 25ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).. Specific staining was localized to connective tissue. Protocol available in COMET™ Panel Builder.​

Detection of Collagen I  alpha 1 in Mouse Kidney.

Collagen I  alpha 1 was detected in perfusion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Mouse Collagen I  alpha 1 Monoclonal Antibody (Catalog # MAB11700) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Collagen I  alpha 1 in Mouse Pancreas.

Collagen I  alpha 1 was detected in perfusion fixed paraffin-embedded sections of mouse pancreas using Rat Anti-Mouse Collagen I  alpha 1 Monoclonal Antibody (Catalog # MAB11700) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Collagen I  alpha 1 in 3T3-L1 cells.

Collagen I  alpha 1 was detected in immersion fixed 3T3‑L1 mouse embryonic fibroblast adipose-like cell line (Positive) and absent in RAW 264.7 mouse monocyte/macrophage cell line (Negative) using Rat Anti-Mouse Collagen I  alpha 1 Monoclonal Antibody (Catalog # MAB11700) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse Collagen I  alpha 1 by Western Blot.

Western Blot shows lysates of MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/ml of Rat Anti-Mouse Collagen I  alpha 1 Monoclonal Antibody (Catalog # MAB11700) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Collagen I  alpha 1 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Mouse Collagen I  alpha 1 by Simple WesternTM.

Simple Western lane view shows lysates of MEF mouse embryonic feeder cells, loaded at 0.1 mg/ml. A specific band was detected for Collagen I  alpha 1 at approximately 226 kDa (as indicated) using 10 µg/ml of Rat Anti-Mouse Collagen I  alpha 1 Monoclonal Antibody (Catalog # MAB11700) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Applications for Mouse Collagen I alpha 1 Antibody (1102924)

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line

Immunohistochemistry

3-25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of mouse kidney and pancreas

Multiplex Immunofluorescence

25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse colon

Simple Western

10 µg/mL
Sample: MEF mouse embryonic feeder cells

Western Blot

1 µg/mL
Sample: MEF mouse embryonic feeder cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Collagen I alpha 1

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides (1). The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues (1). Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described (2). This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

References

  1. Canty, E.G. et al. (2005) J. Cell Sci. 118:1341.
  2. Han, S. et al. (2008) J. Mol. Biol. 383:122.

Alternate Names

COL1A1, OI4

Entrez Gene IDs

1277 (Human); 12842 (Mouse); 29393 (Rat)

Gene Symbol

COL1A1

UniProt

P11087 (Mouse)

Additional Collagen I alpha 1 Products

Product Documents for Mouse Collagen I alpha 1 Antibody (1102924)

Certificate of Analysis

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Product Specific Notices for Mouse Collagen I alpha 1 Antibody (1102924)

For research use only

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Protocols

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