Mouse IL-24 Antibody (303308) [Biotin]

R&D Systems | Catalog # BAM2786

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # 303308
View all IL-24 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL‑24
Gln27-Leu181
Accession # Q925S4.1

Specificity

Detects mouse IL-24 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL‑24 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse IL-24 Antibody (303308) [Biotin]

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑24

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-24

Mouse IL-24, also known as melanoma differentiation-associated gene 7 (MDA-7) and Th2-specific cytokine FISP, is a 27 kDa secreted glycoprotein that is a member of the IL-10 family. It is synthesized as a precursor with a large pro-region that is removed to generate a 155 aa mature protein. Mature mouse IL-24 shares 84% and 69% amino acid identity with rat and human IL-24, respectively.

Long Name

Interleukin 24

Alternate Names

C49A, FISP, IL24, MDA-7, MOB-5, ST16

Entrez Gene IDs

11009 (Human); 93672 (Mouse); 170819 (Rat)

Gene Symbol

IL24

UniProt

Q925S4.1

Additional IL-24 Products

Product Documents for Mouse IL-24 Antibody (303308) [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-24 Antibody (303308) [Biotin]

For research use only

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Protocols

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FAQs

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