Mouse LAMP-1/CD107a Lumenal Domain Antibody

Catalog #: AF4320
18 Citations Datasheet / COA / SDS

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AF4320
AF4320-SP

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Detection of Mouse LAMP‑1/CD107a by Western Blot.

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Citations (18)

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Mouse LAMP-1/CD107a Lumenal Domain Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse LAMP1/CD107a Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant human LAMP1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse LAMP1/CD107a
Leu25-Asn370
Accession # P11438

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse LAMP‑1/CD107a by Western Blot. Western blot shows lysates of mouse small intestine tissue and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse LAMP-1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAMP-1/CD107a at approximately 100-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry

Detection of LAMP-1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

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Detection of LAMP-1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Immunocytochemistry

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LAMP1/CD107a in RAW 264.7 Mouse Cell Line. LAMP1/CD107a was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAMP-1/CD107a

LAMP-1 (lysosome-associated membrane glycoprotein 1; also P2B and CD107a) is a 120 kDa member of the LAMP family of proteins. It is a highly glycosylated type I transmembrane protein whose N-terminus projects either extracellularly or into the lumen of lysosomes. LAMP-1 may protect lysosomal membranes from enzymatic attack or mediate fusion of phagosomes with lysosomes. Mature mouse LAMP1 is 382 amino acids (aa) in length. It contains a 346 aa lumenal domain (aa 25‑370) and a 12 aa cytoplasmic region. Over aa 25‑370, mouse LAMP1 shares 82% and 62% aa identity with rat and human LAMP1, respectively.

Long Name

Lysosome-associated Membrane Glycoprotein 1

Entrez Gene IDs

3916 (Human); 16783 (Mouse)

Alternate Names

CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Mouse LAMP-1/CD107a Lumenal Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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Role of liensinine in sensitivity of activated macrophages to ferroptosis and in acute liver injury
Authors: Jing Li, Qi Huang, Minling Lv, Wenfeng Ma, Jialing Sun, Xin Zhong et al.
Cell Death Discovery
Syntaxin-2 balances phagocytic uptake and phagolysosomal clearance in macrophages
Authors: Samanta, S;Nandi, A;Datta, R;Dolai, S;
Journal of cell science
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Impaired T3 uptake and action in MCT8-deficient cerebral organoids underlie the Allan-Herndon-Dudley syndrome
Authors: Salas-Lucia, F;Escamilla, S;Bianco, AC;Dumitrescu, A;Refetoff, S;
JCI insight
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry
Axonal T3 uptake and transport can trigger thyroid hormone signaling in the brain
Authors: Salas-Lucia F, Fekete C, Sinkó R et al.
eLife
Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival
Authors: J Watson, HR Ferguson, RM Brady, J Ferguson, P Fullwood, H Mo, KH Bexley, D Knight, G Howell, JM Schwartz, MP Smith, C Francavill
Nature Communications, 2022-11-03;13(1):6589.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Ral GTPases are critical regulators of spinal cord myelination and homeostasis
Authors: J DeGeer, AL Datwyler, C Rickenbach, A Ommer, D Gerber, C Fimiani, J Gerber, JA Pereira, U Suter
Cell Reports, 2022-09-27;40(13):111413.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot
Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice
Authors: T Wan, W Zhu, Y Zhao, X Zhang, R Ye, M Zuo, P Xu, Z Huang, C Zhang, Y Xie, X Liu
Nature Communications, 2022-03-03;13(1):1134.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Macrophage autophagy protects mice from cerium oxide nanoparticle-induced lung fibrosis
Authors: Annangi B, Lu Z, Bruniaux J et al.
Particle and Fibre Toxicology
Microglia prevent beta-amyloid plaque formation in the early stage of an Alzheimer's disease mouse model with suppression of glymphatic clearance
Authors: W Feng, Y Zhang, Z Wang, H Xu, T Wu, C Marshall, J Gao, M Xiao
Alzheimers Res Ther, 2020-10-02;12(1):125.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Diurnal Control of Sensory Axon Growth and Shedding in the Mouse Cornea
Authors: S Pal-Ghosh, G Tadvalkar, BA Karpinski, MA Stepp
Invest. Ophthalmol. Vis. Sci., 2020-09-01;61(11):1.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

UNC93B1 recruits syntenin-1 to dampen TLR7 signalling and prevent autoimmunity
Authors: Olivia Majer, Bo Liu, Lieselotte S. M. Kreuk, Nevan Krogan, Gregory M. Barton
Nature

Release from UNC93B1 reinforces the compartmentalized activation of select TLRs
Authors: Olivia Majer, Bo Liu, Brian J. Woo, Lieselotte S. M. Kreuk, Erik Van Dis, Gregory M. Barton
Nature

Lgl1 controls NG2 endocytic pathway to regulate oligodendrocyte differentiation and asymmetric cell division and gliomagenesis
Authors: Mathieu Daynac, Malek Chouchane, Hannah Y. Collins, Nicole E. Murphy, Noemi Andor, Jianqin Niu et al.
Nature Communications

Purkinje Cells Are More Vulnerable to the Specific Depletion of Cathepsin D Than to That of Atg7
Authors: M Koike, M Shibata, T Sunabori, J Yamaguchi, K Sakimura, M Komatsu, K Tanaka, Y Uchiyama
Am. J. Pathol., 2017-05-11;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A
Authors: J Zhang, JL Johnson, J He, G Napolitano, M Ramadass, C Rocca, WB Kiosses, C Bucci, Q Xin, E Gavathioti, AM Cuervo, S Cherqui, SD Catz
J. Biol. Chem., 2017-05-02;292(25):10328-10346.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Intracellular fate of carbon nanotubes inside murine macrophages: pH-dependent detachment of iron catalyst nanoparticles
Authors: Cyrill Bussy, Erwan Paineau, Julien Cambedouzou, Nathalie Brun, Claudie Mory, Barbara Fayard et al.
Particle and Fibre Toxicology

Alterations in Corneal Sensory Nerves During Homeostasis, Aging, and After Injury in Mice Lacking the Heparan Sulfate Proteoglycan Syndecan-1.
Authors: Pal-Ghosh S, Tadvalkar G, Stepp MA.
Invest. Ophthalmol. Vis. Sci.

Frontotemporal dementia nonsense mutation of progranulin rescued by aminoglycosides
Authors: Kuang L, Hashimoto K, Huang EJ et al.
Hum. Mol. Genet.