Mouse LAMP-1/CD107a Lumenal Domain Antibody

R&D Systems | Catalog # AF4320

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LAMP-1/CD107a Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse LAMP1/CD107a
Leu25-Asn370
Accession # P11438

Specificity

Detects mouse LAMP1/CD107a Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant human LAMP1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse LAMP-1/CD107a Lumenal Domain Antibody

Detection of Mouse LAMP-1/CD107a antibody by Western Blot.

Detection of Mouse LAMP‑1/CD107a by Western Blot.

Western blot shows lysates of mouse small intestine tissue and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse LAMP-1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAMP-1/CD107a at approximately 100-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of LAMP-1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of LAMP-1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
LAMP1/CD107a antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC).

LAMP1/CD107a in RAW 264.7 Mouse Cell Line.

LAMP1/CD107a was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of LAMP-1/CD107a by Immunocytochemistry/ Immunofluorescence

Detection of LAMP-1/CD107a by Immunocytochemistry/ Immunofluorescence

Liensinine induces ferritinophagic flux alterations by blocking autophagosome-lysosome fusion in alternatively activated macrophages.a Confocal microscopy images of the co-localization of ferritin (red) with LAMP1 (green), Scale bar = 100 µm (n = 3). b Confocal microscopy images of the co-localization of ferritin (red) with GFP-LC3 (green), Scale bar = 10 µm (n = 3). c Confocal microscopy images of immunostained GFP-LC-3 (green) and LAMP1 (red), Scale bar = 10 µm (n = 3). d Confocal microscopy images of LysoTracker Green (red) and GFP-LC3 (green), Scale bar = 10 µm (n = 3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37353487), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of LAMP-1/CD107a by Immunocytochemistry/ Immunofluorescence

Detection of LAMP-1/CD107a by Immunocytochemistry/ Immunofluorescence

Liensinine induces ferritinophagic flux alterations by blocking autophagosome-lysosome fusion in alternatively activated macrophages.a Confocal microscopy images of the co-localization of ferritin (red) with LAMP1 (green), Scale bar = 100 µm (n = 3). b Confocal microscopy images of the co-localization of ferritin (red) with GFP-LC3 (green), Scale bar = 10 µm (n = 3). c Confocal microscopy images of immunostained GFP-LC-3 (green) and LAMP1 (red), Scale bar = 10 µm (n = 3). d Confocal microscopy images of LysoTracker Green (red) and GFP-LC3 (green), Scale bar = 10 µm (n = 3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37353487), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse LAMP-1/CD107a Lumenal Domain Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

0.2 µg/mL
Sample:   Mouse small intestine tissue and NIH‑3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 2 reviews rated 5 using AF4320 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAMP-1/CD107a

LAMP-1 (lysosome-associated membrane glycoprotein 1; also P2B and CD107a) is a 120 kDa member of the LAMP family of proteins. It is a highly glycosylated type I transmembrane protein whose N-terminus projects either extracellularly or into the lumen of lysosomes. LAMP-1 may protect lysosomal membranes from enzymatic attack or mediate fusion of phagosomes with lysosomes. Mature mouse LAMP1 is 382 amino acids (aa) in length. It contains a 346 aa lumenal domain (aa 25‑370) and a 12 aa cytoplasmic region. Over aa 25‑370, mouse LAMP1 shares 82% and 62% aa identity with rat and human LAMP1, respectively.

Long Name

Lysosome-associated Membrane Glycoprotein 1

Alternate Names

CD107a, LAMP1

Entrez Gene IDs

3916 (Human); 16783 (Mouse)

Gene Symbol

LAMP1

UniProt

P11438

Additional LAMP-1/CD107a Products

Product Documents for Mouse LAMP-1/CD107a Lumenal Domain Antibody

Certificate of Analysis

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Product Specific Notices for Mouse LAMP-1/CD107a Lumenal Domain Antibody

For research use only

Citations for Mouse LAMP-1/CD107a Lumenal Domain Antibody

Customer Reviews for Mouse LAMP-1/CD107a Lumenal Domain Antibody (2)

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Customer Images

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  • Mouse LAMP-1/CD107a Lumenal Domain Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Primary sorted Oligodendrocytes and Primary oligodendrocyte cultures
    Species: Mouse
    Verified Customer | Posted 11/21/2019
    Clean band at predicted molecular weight, 10 ug of protein loaded per lane.
    Dilution of 0.2 mg/mL stock: 1:1000 Great antibody for each application tested so far on mouse tissue and cells: wb, IHC-Fr, ICC
    Mouse LAMP-1/CD107a Lumenal Domain Antibody AF4320
  • Mouse LAMP-1/CD107a Lumenal Domain Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Adult spinal cord and Primary culture of oligodendrocytes
    Species: Mouse
    Verified Customer | Posted 11/21/2019
    P10 mouse ventral funiculus thoracic spinal cord stained with Lamp1 antibody (white) and Dapi.
    Dilution of 0.2 mg/mL stock: 1:40
    Mouse LAMP-1/CD107a Lumenal Domain Antibody AF4320

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