Mouse/Rat IL-10 Antibody

Catalog # Availability Size / Price Qty
AF519
AF519-SP
Mouse/Rat IL-10 Antibody in Data
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Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (4)

Mouse/Rat IL-10 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse, rat, and cotton rat IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human IL-10, recombinant guinea pig IL-10, recombinant viral IL-10 and recombinant canine IL-10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat IL‑10
Ser19-Asn178
Accession # P29456
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed rat splenocytes
Neutralization
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 40 ng/mL Recombinant Rat IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Recombinant Mouse and Rat IL‑10 by Western Blot. View Larger

Detection of Recombinant Mouse and Rat IL‑10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL-10 (Catalog # 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Neutralization Cell Proliferation Induced by IL‑10 and Neutralization by Rat IL‑10 Antibody. View Larger

Cell Proliferation Induced by IL‑10 and Neutralization by Rat IL‑10 Antibody. Recombinant Rat IL-10 (Catalog # 522-RL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519). The ND50is typically 0.3-1.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL‑22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature rat IL‑10 shares 85% amino acid sequence identity with mouse and 71%‑79% amino acid sequence identity with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8‑10). The IL-10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL‑10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12‑14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15‑17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

References
  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
  3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
  4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
  5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
  6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
  7. Hsu, D.-H. et al. (1990) Science 250:830.
  8. Goodman, R. et al. (1992) Biochem. Biophys. Res. Commun. 189:1.
  9. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
  10. Syto, R. et al. (1998) Biochemistry 37:16943.
  11. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  12. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  13. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  14. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  15. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  16. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
  17. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.
Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Product Datasheets

Citations for Mouse/Rat IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Activation of GPR40 produces mechanical antiallodynia via the spinal glial interleukin-10/?-endorphin pathway
    Authors: XF Mao, HY Wu, XQ Tang, U Ali, H Liu, YX Wang
    J Neuroinflammation, 2019;16(1):84.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury
    Authors: S Zilberman-, R Abu-Hamad, R Zarura, M Sova, Y Hachmo, M Stark, S Neuman, S Slavin, S Efrati
    PLoS ONE, 2019;14(9):e0222354.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Fluorescence Microscopy
  3. Involvement of Interleukin-10 in Analgesia of Electroacupuncture on Incision Pain
    Authors: WJ Dai, JL Sun, C Li, W Mao, YK Huang, ZQ Zhao, YQ Zhang, N Lü
    Evid Based Complement Alternat Med, 2019;2019(0):8413576.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Blocking p38 Signaling Reduces the Activation of Pro-inflammatory Cytokines and the Phosphorylation of p38 in the Habenula and Reverses Depressive-Like Behaviors Induced by Neuroinflammation
    Authors: YW Zhao, YQ Pan, MM Tang, WJ Lin
    Front Pharmacol, 2018;9(0):511.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury
    Authors: Y Yang, H Liu, H Zhang, Q Ye, J Wang, B Yang, L Mao, W Zhu, RK Leak, B Xiao, B Lu, J Chen, X Hu
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Autocrine interleukin-10 mediates glucagon-like peptide-1 receptor-induced spinal microglial ?-endorphin expression
    Authors: HY Wu, XQ Tang, XF Mao, YX Wang
    J. Neurosci., 2017;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN?
    Authors: JE Lim, E Chung, Y Son
    Sci Rep, 2017;7(1):9417.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization
    Authors: M Viladomiu, J Bassaganya, N Tubau-Juni, B Kronsteine, A Leber, CW Philipson, V Zoccoli-Ro, R Hontecilla
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. Y RNA fragment in extracellular vesicles confers cardioprotection via modulation of IL-10 expression and secretion
    Authors: L Cambier, G de Couto, A Ibrahim, AK Echavez, J Valle, W Liu, M Kreke, RR Smith, L Marbán, E Marbán
    EMBO Mol Med, 2017;9(3):337-352.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Cerebral regulatory T cells restrain microglia/macrophage-mediated inflammatory responses via IL-10.
    Authors: Xie L, Choudhury G, Winters A, Yang S, Jin K
    Eur J Immunol, 2015;45(1):180-91.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Immunization with a Myelin-Derived Antigen Activates the Brain's Choroid Plexus for Recruitment of Immunoregulatory Cells to the CNS and Attenuates Disease Progression in a Mouse Model of ALS.
    Authors: Kunis G, Baruch K, Miller O, Schwartz M
    J Neurosci, 2015;35(16):6381-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. CNS repair requires both effector and regulatory T cells with distinct temporal and spatial profiles.
    Authors: Raposo C, Graubardt N, Cohen M, Eitan C, London A, Berkutzki T, Schwartz M
    J Neurosci, 2014;34(31):10141-55.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Serum-starved adipose-derived stromal cells ameliorate crescentic GN by promoting immunoregulatory macrophages.
    Authors: Furuhashi, Kazuhiro, Tsuboi, Naotake, Shimizu, Asuka, Katsuno, Takayuki, Kim, Hangsoo, Saka, Yosuke, Ozaki, Takenori, Sado, Yoshikaz, Imai, Enyu, Matsuo, Seiichi, Maruyama, Shoichi
    J Am Soc Nephrol, 2013;24(4):587-603.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  14. Functionalized scaffold-mediated interleukin 10 gene delivery significantly improves survival rates of stem cells in vivo.
    Authors: Holladay C, Power K, Sefton M, O'Brien T, Gallagher WM, Pandit A
    Mol. Ther., 2011;19(5):969-78.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Ocular regulatory T cells distinguish monophasic from recurrent autoimmune uveitis.
    Authors: Ke Y, Jiang G, Sun D, Kaplan HJ, Shao H
    Invest. Ophthalmol. Vis. Sci., 2008;49(9):3999-4007.
    Species: Rat
    Sample Types: Aqueous Humor
    Applications: Neutralization

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Reviews for Mouse/Rat IL-10 Antibody

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Mouse/Rat IL-10 Antibody
By Anonymous on 11/30/2020
Application: WB Sample Tested: Bronchoalveolar lavage Species: Rat

We had an issue with shipping due to COVID, that caused the antibody to sit at room temperature for too long and become unusable. Fortunately we placed another order of the exact same product number, and it performed perfectly. I've included the photo of the gel after the shipping mishap


Mouse/Rat IL-10 Antibody
By Anonymous on 11/30/2020
Application: WB Sample Tested: Control Species: Rat

This image was purely a test with an IL-10 control protein at different concentrations. Initially we were unable to detect the IL-10 protein in our samples, so we were testing to ensure that this antibody was functional. Turns out it was!


Mouse/Rat IL-10 Antibody
By Anonymous on 04/24/2018
Application: IHC Sample Tested: Spleen tissue Species: Mouse

Mouse/Rat IL-10 Antibody
By Anonymous on 07/27/2016
Application: B/N Sample Tested: Splenic B cells Species: Mouse