Rat IL-10 Antibody

Catalog # Availability Size / Price Qty
MAB519-SP
MAB519-100
MAB519-500
Rat IL-10 Antibody in Data
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Rat IL-10 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IL-10 in ELISAs and Western blots. In Western blots, shows approximately 15% cross-reactivity with recombinant mouse IL‑10 and less than 2% cross-reactivity with recombinant human IL-10.
Source
Monoclonal Mouse IgG2B Clone # 67232
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat IL‑10
Ser19-Asn178
Accession # P29456
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Rat IL-10 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Rat IL‑10 Biotinylated Antibody (Catalog # BAF519)

Standard: Recombinant Rat IL-10 Protein (Catalog # 522-RL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Recombinant Mouse and Rat IL-10 antibody by Western Blot. View Larger

Detection of Recombinant Mouse and Rat IL‑10 by Western Blot. Western blot shows 25 ng of Recombinant Rat IL-10 (Catalog # 522-RLB), Recombinant Human IL-10 (Catalog # 217-IL) and Recombinant Mouse IL-10 (Catalog # 417-ML). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Rat IL-10 Monoclonal Antibody (Catalog # MAB519) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Product Datasheets

Citations for Rat IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. The role of cyclosporine and mycophenolate in an orthotopic porcine-to-rat corneal xenotransplantation.
    Authors: Lee HI, Kim MK, Oh JY, Ko JH, Lee HJ, Wee WR, Lee JH
    J. Korean Med. Sci., 2008;23(3):492-501.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  2. The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury.
    Authors: Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH
    Stem Cells, 2008;26(4):1047-55.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  3. In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
    Authors: Roche M, Diamond M, Finn DP
    J. Neuroimmunol., 2006;181(1):57-67.
    Species: Rat
    Sample Types: Plasma
    Applications: ELISA Development

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Rat IL-10 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Rat IL-10 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Rat IL-10 Antibody
By Anonymous on 11/08/2017
Application: ELISA Sample Tested: Serum Species: Rat

This antibody was used to build an ELISA to measure rat IL-10. This antibody worked well as a capture antibody and BAF519 as the detection antibody.