Mouse Syndecan‑1/CD138 Alexa Fluor™ Plus 680‑conjugated Antibody

Name: Mouse Syndecan‑1/CD138 Alexa Fluor™ Plus 680‑conjugated Antibody
Brand: R&D Systems
SKU: FAB29661AFP680
Price: 549 USD
Availability: InStock

R&D Systems | Catalog # FAB29661AFP680

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Rat IgG₁ Clone # 320611

View all Syndecan-1/CD138 Primary Antibodies »

Product Specifications

Specificity

Detects mouse Syndecan‑1/CD138 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Syndecan-1, recombinant mouse (rm) Syndecan-2, rmSyndecan-3, or rmSyndecan-4 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Applications for Mouse Syndecan‑1/CD138 Alexa Fluor™ Plus 680‑conjugated Antibody

Application

Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Syndecan-1/CD138

Syndecan-1, designated CD138, is a dimeric type I transmembrane (TM) protein that belongs to the Syndecan family of Type 1 transmembrane proteins (1, 2). The four Syndecan family members are major carriers of heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans (GAGs) that have different expression patterns and extracellular sequences. Syndecan-1 forms weak non-covalent homodimers, or heterodimers with Syndecan-2 or -3, through interactions of the transmembrane domain (3). It is synthesized as a 310 amino acid (aa) precursor with a 22 aa signal sequence, a 233 aa extracellular domain (ECD) that includes three closely spaced consensus Ser-Gly HS attachment sites near the N-terminus, a 21 aa TM segment, and a 35 aa cytoplasmic region that includes a PDZ binding motif with a tyrosine phosphorylation site (4). The ECD is variably modified by GAGs, producing molecular weights of 120‑200 kDa for native Syndecan-1. Soluble forms are shed via proteolytic cleavage. Mouse Syndecan-1 ECD shares 70% and 87% aa identity with the ECD of human and rat Syndecan-1, respectively. Alternative splicing in mouse generates an isoform with an internal deletion of 44 aa from the ECD (5). Syndecan-1 shows highest expression on epithelial cells such as keratinocytes, and terminally differentiated B cells such as plasma cells (6, 7). It aids wound healing in skin, cornea, and heart following myocardial infarction by promoting re-epithelialization, migration, and collagen deposition (6‑10). It binds chemokines, creating chemotactic gradients when shed, but also binds and modulates integrins to control the influx of leukocytes (7, 9, 11). The net effect is to allow, but limit, inflammation. In myeloma and other cancers, shedding of Syndecan-1 can facilitate growth, angiogenesis and metastasis (12‑14). Growth factors, such as FGFs and HGF, bind GAG chains and use Syndecan-1 as a coreceptor (14, 15). The GAG chains may also be used by a variety of viruses and bacteria for cell adhesion and uptake (6).

References

Tkachenko, E. et al. (2005) Circ. Res. 96:488.
Mali, M. et al. (1990) J. Biol. Chem. 265:6884.
Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
Saunders, S. et al. (1989) J. Cell Biol. 108:1547.
Romaris, M. et al. (1999) J. Biol. Chem. 274:18667.
Fears, C.Y. and A. Woods (2006) Matrix Biol. 25:443.
Stepp, M.A. et al. (2002) J. Cell Sci. 115:4517.
Ojeh, N. et al. (2008) J. Invest. Dermatol. 128:26.
Stepp, M.A. et al. (2007) J. Cell Sci. 120:2851.
Vanhoutte, D. et al. (2007) Circulation 115:475.
Li, Q. et al. (2002) Cell 111:635.
Beauvais, D.M. et al. (2009) J. Exp. Med. 206:691.
Yang, Y. et al. (2007) J. Biol. Chem. 282:13326.
Derksen, P.W.B. et al. (2002) Blood 99:1405.
Su, G. et al. (2007) J. Biol. Chem. 282:14906.

Alternate Names

CD138, SDC1, Syndecan1

Entrez Gene IDs

6382 (Human); 20969 (Mouse); 100519770 (Porcine); 100338470 (Rabbit)

Gene Symbol

SDC1

UniProt

P18828

Additional Syndecan-1/CD138 Products

Product Documents for Mouse Syndecan‑1/CD138 Alexa Fluor™ Plus 680‑conjugated Antibody

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Product Specific Notices for Mouse Syndecan‑1/CD138 Alexa Fluor™ Plus 680‑conjugated Antibody

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only