Mouse UTF1 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF7819G Datasheet / COA / SDS

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AF7819G-100UG

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Mouse UTF1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse UTF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human UTF1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse UTF1
Lys50-Ser171
Accession # Q6J1H4

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: UTF1

UTF1 (Undifferentiated embryonic cell Transcription Factor 1) is a 47-49 kDa member of Leu-zipper family of proteins. It is expressed in the early embryonic inner cell mass and primitive ectoderm, and disappears following the formation of the primitive streak. In this regard, UTF1 has been shown to be chromatin-associated and to participate in the proper differentiation of embryonic stem cells. It also is expressed in basal keratinocytes, and hair follicle matrix cells. UTF1 acts as a transcriptional coactivator of ATF2 (Activating Transcription factor 2), a bZIP family protein that interacts with CRE in gene promoters. Mouse UTF1 is 399 amino acids (aa) in length. It contains two conserved domains (aa 52-167 and aa 269-332), the latter which is key to ATS2 activation. There is one alternative start site at Met43 that generates a 39 kDa isoform. Over aa 18-423, mouse UTF1 shares 81% and 95% aa sequence identity with human and rat UTF1, respectively.

Long Name

Undifferentiated Embryonic Cell Transcription Factor 1

Entrez Gene IDs

8433 (Human); 22286 (Mouse); 681901 (Rat)

Alternate Names

undifferentiated embryonic cell transcription factor 1; UTF1